CAJ | 학술논문

目的：本研究旨在分析和比较中国荷斯坦牛、娟姗牛和水牛的乳蛋白基因多态性。方法根据奶牛多态位点设计引物,采用焦磷酸测序法分析乳蛋白基因多态性,并采用高效液相色谱法进行验证。结果荷斯坦牛和娟姗牛的乳蛋白(β-casein,κ-casein和β-lactoglobulin)均存在基因多态性,而水牛仅在κ-casein上存在多态性且多态位点与另两种奶牛不同。结论中国荷斯坦牛、娟姗牛和水牛的乳蛋白基因均存在多态性,焦磷酸测序法能高通量、快速测定乳蛋白基因多态性。
목적：본연구지재분석화비교중국하사탄우、연산우화수우적유단백기인다태성。방법근거내우다태위점설계인물,채용초린산측서법분석유단백기인다태성,병채용고효액상색보법진행험증。결과하사탄우화연산우적유단백(β-casein,κ-casein화β-lactoglobulin)균존재기인다태성,이수우부재κ-casein상존재다태성차다태위점여령량충내우불동。결론중국하사탄우、연산우화수우적유단백기인균존재다태성,초린산측서법능고통량、쾌속측정유단백기인다태성。
ObjectiveTo analyze and compare the genetic polymorphisms of milk protein in Chinese Holstein cattle, Jersey cattle and water buffalo.MethodsThe primers were designed according to the milk protein polymorphic sites of Holstein, and the genetic polymorphisms were analyzed by pyrosequencing and confirmed by RP-HPLC.ResultsGenetic polymorphisms of milk protein genes (β-casein,κ-casein andβ-lactoglobulin) were detected in Chinese Holstein cattle and Jersey cattle. However, no polymorphism was found in water buffalo exceptκ-casein, and the polymorphic site was different with Holstein and Jersey. ConclusionMilk protein polymorphism was found in Chinese Holstein, Jersey and buffalo, and pyrose-quencing was a high-throughput and fast method for milk protein genotyping.