CAJ | 학술논문

纤维素酶是来源于真菌、细菌和原生动物并且能够降解纤维素成为葡萄糖单体的一组酶的总称,包括内切型(β-l,4)葡聚糖水解酶、外切型(β-1,4)葡聚糖水解酶、β-葡萄糖苷酶。β-葡萄糖苷酶能够水解纤维二糖产生两分子的葡萄糖,是纤维素酶的限速酶,但其含量少、活力低,成为纤维素酶解的瓶颈。因此,通过基因重组技术构建工程菌,开展关于β-葡萄糖苷酶基因高效表达的研究具有重要的意义。本文从β-葡萄糖苷酶及其菌株的筛选和育种、β-葡萄糖苷酶基因的克隆与表达、分泌及诱导等方面论述了如何使β-葡萄糖苷酶在丝状真菌中得到高效表达。
섬유소매시래원우진균、세균화원생동물병차능구강해섬유소성위포도당단체적일조매적총칭,포괄내절형(β-l,4)포취당수해매、외절형(β-1,4)포취당수해매、β-포도당감매。β-포도당감매능구수해섬유이당산생량분자적포도당,시섬유소매적한속매,단기함량소、활력저,성위섬유소매해적병경。인차,통과기인중조기술구건공정균,개전관우β-포도당감매기인고효표체적연구구유중요적의의。본문종β-포도당감매급기균주적사선화육충、β-포도당감매기인적극륭여표체、분비급유도등방면논술료여하사β-포도당감매재사상진균중득도고효표체。
Cellulase is a group of several enzymes produced chiefly by fungi, bacteria, and protozoans which can catalyze cellulolysis, the decomposition of cellulose. It includes endo-1,4-beta-D-glucanase (beta-1, 4-glucanase, beta-1,4-endoglucan hydrolase, endoglucanase D, 1,4-(1,3,1,4)-beta-D-glucan 4-glucanohydrolase) andβ-glucosidase.β-glucosidase hydrolyzed cellobiose into 2 molecules of glucose which is the rate-limiting enzyme of cellulase. Moreover,β-glucosidase creates a bottleneck in cellulase hydrolysis owing to low content and poor enzyme activity. It is important to study the high level expression ofβ-glucosidase gene by construct-ing of engineering bacteria using gene recombination technology. Herein, we review the current progress of studies on the strain screening and breeding,β-glucosidase gene cloning, secretion and induction, and high lev-el expression in filamentous fungi.