CAJ | 학술논문

目的：建立馅料中二氧化硫脲的超高效液相色谱测定方法。方法用0.05%乙酸水溶液提取,样品提取液经过离心,乙腈稀释过滤膜过滤后,以 HILIC 色谱柱为分析柱分离,采用超高效液相色谱-二极管阵列检测器(ultra performance liquid chromatography-photo-diode array, UPLC-PDA)检测,以乙腈和水为流动相,梯度洗脱,检测波长为284 nm。采用外标法定量。结果二氧化硫脲的线性范围为0.4~20.0 mg/L,相关系数不小于0.999,空白样品的加标回收的回收率为80.6%~102.2%,相对标准偏差为2.6%~5.0%。结论该方法简便、快速、准确、重现性好,适用于馅料中二氧化硫脲的检测。
목적：건립함료중이양화류뇨적초고효액상색보측정방법。방법용0.05%을산수용액제취,양품제취액경과리심,을정희석과려막과려후,이 HILIC 색보주위분석주분리,채용초고효액상색보-이겁관진렬검측기(ultra performance liquid chromatography-photo-diode array, UPLC-PDA)검측,이을정화수위류동상,제도세탈,검측파장위284 nm。채용외표법정량。결과이양화류뇨적선성범위위0.4~20.0 mg/L,상관계수불소우0.999,공백양품적가표회수적회수솔위80.6%~102.2%,상대표준편차위2.6%~5.0%。결론해방법간편、쾌속、준학、중현성호,괄용우함료중이양화류뇨적검측。
Objective To establish a method to detect the content of thiourea dioxide in fillings of bakery food by using ultra performance liquid chromatography (UPLC).MethodsThe thiourea dioxide was ex-tracted by 0.05% acetic acid, and then centrifuged. The extracts was separated by HILIC column, and detected by the photo-diode array (PDA) at the wavelength of 284 nm. ResultsThe thiourea dioxide concentration and the peak area showed a good linearity from 0.4 to 20 mg/L with the correlation (r) no less than 0.999. The av-erage recoveries of the spiked blank fillings of bakery food were from 80.6% to 102.2% with the relative stan-dard deviations (RSD) in the range of 2.6%~5.0%.ConclusionThe method was simple, quick, accurate and robust for the detection of thiourea dioxide in fillings of bakery food.