CAJ | 학술논문

[目的]仔猪在14日龄接种蓝耳病毒疫苗，25日龄接种猪瘟疫苗，检测接种蓝耳病疫苗和猪瘟疫苗后的免疫合格率。[方法]采用猪繁殖与呼吸综合征病毒和猪瘟病毒ELISA抗体检测试剂盒检测抗体表达量。[结果]猪蓝耳病疫苗免疫3 d后，6.7%免疫个体为抗体阳性，免疫7 d后，26.7%免疫个体为抗体阳性，免疫14 d后，85%免疫个体为抗体阳性，免疫后30 d 100%免疫个体为抗体阳性；猪瘟疫苗免疫3 d后，13%免疫个体为抗体阳性，免疫7 d后，40%免疫个体为抗体阳性，免疫14 d后，58.3%免疫个体为抗体阳性，免疫30 d后，83.3%免疫个体为抗体阳性。[结论]仔猪在蓝耳病疫苗免疫3d后抗体效价最低，30 d后免疫效果很好，接种猪瘟疫苗3d后抗体效价最低，仔猪不足以抵抗病毒侵袭，虽然蓝耳病疫苗和猪瘟疫苗接种后30 d，抗体水平都达到了农业部规定的70%的标准，但是猪瘟疫苗免疫效果不能达到100%理想效果。
[목적]자저재14일령접충람이병독역묘，25일령접충저온역묘，검측접충람이병역묘화저온역묘후적면역합격솔。[방법]채용저번식여호흡종합정병독화저온병독ELISA항체검측시제합검측항체표체량。[결과]저람이병역묘면역3 d후，6.7%면역개체위항체양성，면역7 d후，26.7%면역개체위항체양성，면역14 d후，85%면역개체위항체양성，면역후30 d 100%면역개체위항체양성；저온역묘면역3 d후，13%면역개체위항체양성，면역7 d후，40%면역개체위항체양성，면역14 d후，58.3%면역개체위항체양성，면역30 d후，83.3%면역개체위항체양성。[결론]자저재람이병역묘면역3d후항체효개최저，30 d후면역효과흔호，접충저온역묘3d후항체효개최저，자저불족이저항병독침습，수연람이병역묘화저온역묘접충후30 d，항체수평도체도료농업부규정적70%적표준，단시저온역묘면역효과불능체도100%이상효과。
Objective] The passing rates of porcine reproductive and respiratory syn-drome virus (PRRSV) vaccine and classical swine fever virus (CSFV) vaccine were investigated in this paper. The PRRSV vaccine was injected in 14-d-old piglets and the CSFV vaccine was injected in 25-d-old piglets. [Method] The antibody expres-sion amounts were detected with the PRRSV antibody ELlSA detection kit and the CSFV antibody ELlSA detection kit. [Result] The antibody positive rates were 6.7%, 26.7%, 85% and 100% 3, 7, 14 and 30 d after the vaccination of PRRSV vaccine. While for the CSFV vaccine, 13%, 40%, 58.3% and 83.3% of the immune individu-als were antibody positive 3, 7, 14 and 30 d after the vaccination. [Conclusion] The PRRSV vaccine showed the lowest antibody tilter 3 d after the inoculation, but had a great vaccination effect 30 d after the inoculation. The piglets were not immune enough against viral invasion 3 d after the inoculation of CSFV vaccine. Although the antibody positive rate of PRRSV and CSFV vaccines al reached the required level by the Ministry of Agriculture (70%) 30 d after the inoculation, the vaccination of CSFV vaccine could not achieve the ideal effect of 100%.