农业科学与技术(英文版)
農業科學與技術(英文版)
농업과학여기술(영문판)
AGRICULTURAL SCIENCE & TECHNOLOGY
2014年
10期
1671-1675
,共5页
李畅%苏家乐%刘晓青%陈尚平%何丽斯
李暢%囌傢樂%劉曉青%陳尚平%何麗斯
리창%소가악%류효청%진상평%하려사
一品红%雌花%柱头%形态发育%可授性
一品紅%雌花%柱頭%形態髮育%可授性
일품홍%자화%주두%형태발육%가수성
Euphorbia pulcherrima%Pistil ate flower%Stigma%Morphological develop-ment%Receptivity
[目的]该研究指在探究一品红雌花柱头发育形态与柱头可授性的关系。[方法]以一年生一品红‘千禧’为材料,对其雌花发育进行了宏观及扫描电镜(SEM)观察,并采用联苯胺-过氧化氢法和活体花粉萌发法检测柱头可授性。[结果]一品红雌花发育进程可分为显花期、初开期、Y字期、八字期和翻卷期5个时期。雌花发育与柱头可授性具有相关性:柱头可授期在柱头裂片微张(初开期)和柱头开始翻卷(八字期)之间,最佳授粉期为八字期,持续时间3~5 d,柱头倒八状, SEM观察柱头表面可见大量渗出物;采用联苯胺-过氧化氢法检测柱头不同形态时期的可授性结果与活体花粉萌发法基本吻合。[结论]该研究表明在育种实践中,可根据雌花柱头发育形态来判别一品红最佳授粉时期。
[目的]該研究指在探究一品紅雌花柱頭髮育形態與柱頭可授性的關繫。[方法]以一年生一品紅‘韆禧’為材料,對其雌花髮育進行瞭宏觀及掃描電鏡(SEM)觀察,併採用聯苯胺-過氧化氫法和活體花粉萌髮法檢測柱頭可授性。[結果]一品紅雌花髮育進程可分為顯花期、初開期、Y字期、八字期和翻捲期5箇時期。雌花髮育與柱頭可授性具有相關性:柱頭可授期在柱頭裂片微張(初開期)和柱頭開始翻捲(八字期)之間,最佳授粉期為八字期,持續時間3~5 d,柱頭倒八狀, SEM觀察柱頭錶麵可見大量滲齣物;採用聯苯胺-過氧化氫法檢測柱頭不同形態時期的可授性結果與活體花粉萌髮法基本吻閤。[結論]該研究錶明在育種實踐中,可根據雌花柱頭髮育形態來判彆一品紅最佳授粉時期。
[목적]해연구지재탐구일품홍자화주두발육형태여주두가수성적관계。[방법]이일년생일품홍‘천희’위재료,대기자화발육진행료굉관급소묘전경(SEM)관찰,병채용련분알-과양화경법화활체화분맹발법검측주두가수성。[결과]일품홍자화발육진정가분위현화기、초개기、Y자기、팔자기화번권기5개시기。자화발육여주두가수성구유상관성:주두가수기재주두렬편미장(초개기)화주두개시번권(팔자기)지간,최가수분기위팔자기,지속시간3~5 d,주두도팔상, SEM관찰주두표면가견대량삼출물;채용련분알-과양화경법검측주두불동형태시기적가수성결과여활체화분맹발법기본문합。[결론]해연구표명재육충실천중,가근거자화주두발육형태래판별일품홍최가수분시기。
Objective] This study aimed to investigate the relationship between pistil-late flower developmental morphology and stigma receptivity in Euphorbia pulcherri-ma. [Method] One-year-old adult plants of E. pulcherrima cultivar ‘Mil enium’ were used as experimental materials to analyze pistil ate flower development process by macroscopic and scanning electron microscope (SEM) observation. Stigma receptivity was detected with benzidine-hydrogen peroxide method and in vivo pol en germina-tion method. [Result] The pistil ate flower development process of E. pulcherrima was consisted of columnar stigma phase, stigma lobe slightly opening phase, Y-shape stigma lobe phase, inverse V-shaped stigma lobe phase and stigma curling phase. Pistil ate flower development and stigma receptivity of E. pulcherrima exhibit-ed certain correlation. The stigma receptive period lasted from stigma lobe slightly opening phase to inverse V-shaped stigma lobe phase; inverse V-shaped stigma lobe phase was appropriate for pol ination, lasting for 3-5 d, with V-shaped stigma lobe and a large amount of exudates on stigma surface according to SEM obser-vation. Stigma receptivity of E. pulcherrima detected with benzidine-hydrogen perox-ide method was consistent with that detected in vivo pol en germination method. [Conclusion] ln breeding practice, the optimal pol ination period of E. pulcherrima can be determined based on the developmental morphology of pistil ate flower.