CAJ | 학술논문

为建立贵州续断药材的指纹图谱，采用 D101大孔树脂对样品进行前处理，用 Agilent ZORB-AX extend C18（250 mm×4．6 mm，5μm）色谱柱进行梯度洗脱，采用 SPSS19．0进行数据分析，对贵州不同产地的续断药材进行指纹图谱构建。结果表明：11批续断样品有14个共有峰，方法学考察符合指纹图谱技术要求；聚类分析聚为2类，贵州不同产地续断药材指纹图谱相似度极高，建立的续断指纹图谱测定方法准确可靠。
위건립귀주속단약재적지문도보，채용 D101대공수지대양품진행전처리，용 Agilent ZORB-AX extend C18（250 mm×4．6 mm，5μm）색보주진행제도세탈，채용 SPSS19．0진행수거분석，대귀주불동산지적속단약재진행지문도보구건。결과표명：11비속단양품유14개공유봉，방법학고찰부합지문도보기술요구；취류분석취위2류，귀주불동산지속단약재지문도보상사도겁고，건립적속단지문도보측정방법준학가고。
In order to establish the chromatographic fingerprint of Dipsacus asperoides produced from Guizhou,samples were pre-treated by D101macroporous resin,agilent ZORBAX extend C18 (250 mm 4.6 mm,5 μm)column was used for gradient elution,and SPSS 19.0 was used for data analysis.The results showed that the 11 batches of D.asperoides had 14 common peaks,the methodological study results met the requirements of chromatographic fingerprint.According to system clustering analysis,it could be divided into 2 categories. The similarity of chromatographic fingerprint among D.asperoides from different origins in Guizhou was extremely high.Therefore,the determination method in the study could be approved accurate and reliable.