CAJ | 학술논문

以水为溶剂，采用微波辅助法提取赤芍活性成分；以最大紫外吸收波长处的吸光度为评价指标，通过单因素实验、Box-Behnken设计和响应曲面法考察了提取温度、提取时间、液料比等对提取效果的影响，对赤芍的提取工艺进行了优化分析；采用毛细管电泳法对所得赤芍水提液进行分离检测，并对不同产地赤芍的活性成分含量进行了比较分析。确定赤芍活性成分的最优提取条件为：提取温度89.99℃、提取时间75.32 min、液料比79.25∶1（mL∶g），在此条件下，赤芍水提液的吸光度预测值为0.3022，实测值为0.2899，表明该方法预测效果良好，可为中药活性成分的微波辅助提取提供参考。
이수위용제，채용미파보조법제취적작활성성분；이최대자외흡수파장처적흡광도위평개지표，통과단인소실험、Box-Behnken설계화향응곡면법고찰료제취온도、제취시간、액료비등대제취효과적영향，대적작적제취공예진행료우화분석；채용모세관전영법대소득적작수제액진행분리검측，병대불동산지적작적활성성분함량진행료비교분석。학정적작활성성분적최우제취조건위：제취온도89.99℃、제취시간75.32 min、액료비79.25∶1（mL∶g），재차조건하，적작수제액적흡광도예측치위0.3022，실측치위0.2899，표명해방법예측효과량호，가위중약활성성분적미파보조제취제공삼고。
Using water as solvent,active ingredients of Radix Paeoniae Rubra were extracted by microwave-assisted extraction method.Using UV absorbance at maximum absorption wavelength as evaluation index,the influences of extraction temperature,extraction time,liquid-solid ratio on extraction efficiency were investigated by single factor experiment,Box-Behnken design and response surface methodology(RSM),and the extraction technology was optimized.Capillary electrophoresis was used for separation and detection of Radix Paeoniae Rubra extract.The contents of active ingredients of Radix Paeoniae Rubra from different areas were compared. The optimum extraction conditions were obtained as follows:extraction temperature of 89.99 ℃,extraction time of 75.32 min,liquid-solid ratio of 79.25∶1(mL∶g).Under above conditions,the predicted and measured value of UV absorbance were 0.3022,0.2899,respectively.This method can provide references for the micro-wave-assisted extraction of active ingredients of Chinese medicine.