CAJ | 학술논문

选取机体Cu（Ⅱ）浓度，运用衰减全反射傅立叶变换红外光谱（ATR-FTIR）、SDS-PAGE电泳和冯克劳斯法，分析了Cu（Ⅱ）对过氧亚硝酸根介导的硝化反应中纤维蛋白原二级结构和凝聚活性的影响。结果显示：随着 Cu（Ⅱ）浓度的增加，硝化纤维蛋白原中110 kDa新增条带越来越清晰；二级结构中α-螺旋比例下降，β-折叠比例上升；凝聚活性逐渐降低；当Cu（Ⅱ）浓度为1.66 mmol·L-1时，硝化纤维蛋白原中的α-螺旋比例由最初的32.29％减少到25.15％，β-折叠比例从35.66％升高到44.47％；冯克劳斯法检测硝化纤维蛋白原失去凝聚能力，但 SDS-PAGE电泳表明其依然可以释放纤维蛋白单体。表明，Cu（Ⅱ）能显著影响硝化反应中纤维蛋白原的二级结构，抑制其凝聚活性，促进硝化损伤。
선취궤체Cu（Ⅱ）농도，운용쇠감전반사부립협변환홍외광보（ATR-FTIR）、SDS-PAGE전영화풍극로사법，분석료Cu（Ⅱ）대과양아초산근개도적초화반응중섬유단백원이급결구화응취활성적영향。결과현시：수착 Cu（Ⅱ）농도적증가，초화섬유단백원중110 kDa신증조대월래월청석；이급결구중α-라선비례하강，β-절첩비례상승；응취활성축점강저；당Cu（Ⅱ）농도위1.66 mmol·L-1시，초화섬유단백원중적α-라선비례유최초적32.29％감소도25.15％，β-절첩비례종35.66％승고도44.47％；풍극로사법검측초화섬유단백원실거응취능력，단 SDS-PAGE전영표명기의연가이석방섬유단백단체。표명，Cu（Ⅱ）능현저영향초화반응중섬유단백원적이급결구，억제기응취활성，촉진초화손상。
The concentration of Cu(Ⅱ)was selected in biomimetic system to analyze the influence of Cu(Ⅱ) on secondary structure changes and aggregation activity of fibrinogen in nitration reaction by peroxynitrite with the aid of ATR-FTIR,SDS-PAGE and Von Clauss method.The results showed that with the increase of Cu(Ⅱ) concentration,a 110 kDa band became more and more clearer,theα-helix proportion declined whileβ-fold pro-portion increased in the secondary structure of nitration fibrinogen,and aggregation activity decreased gradual-ly.When Cu(Ⅱ)concentration increased to 1.66 mmol·L-1,theα-helix proportion decreased from initial 32.29% to 25.15%,whileβ-fold proportion increased from 35.66% to 44.47%.The nitration fibrinogen lost aggregation activity as detected by Von Clauss method,though SDS-PAGE showed that it still could release fi-brin monomer.The research demonstrated that Cu(Ⅱ)could significantly influence the fibrinogen secondary structure changes during nitration reaction,inhibit its aggregation activity and promote nitration inj ury.