化学与生物工程
化學與生物工程
화학여생물공정
CHEMISTRY & BIOENGINEERING
2014年
10期
14-19
,共6页
邻菲罗啉衍生物%苯并咪唑%合成%光谱%DNA%生物活性
鄰菲囉啉衍生物%苯併咪唑%閤成%光譜%DNA%生物活性
린비라람연생물%분병미서%합성%광보%DNA%생물활성
phenanthroline derivatives%benzimidazole%synthesis%spectroscopy%DNA%bioactivity
合成了3个新的2-（4-羟苯基）咪唑并[4，5-f]1，10-邻菲罗啉衍生物，通过红外光谱、质谱、1 HNMR、元素分析等对其结构进行了表征，并测定了其紫外和荧光光谱性质及生物活性。结果表明：3个化合物均具有较强的荧光和较大的斯托克斯位移，对双链DNA（AT、GC）和 G-四链体DNA（Hum24）均有一定的相互作用；DNA的加入能使3个化合物的荧光发生猝灭，其中荧光猝灭程度与双链DNA（AT、GC）的浓度呈线性关系。据此可将这3个化合物开发用作荧光猝灭探针以检测双链DNA（AT、GC）的浓度。
閤成瞭3箇新的2-（4-羥苯基）咪唑併[4，5-f]1，10-鄰菲囉啉衍生物，通過紅外光譜、質譜、1 HNMR、元素分析等對其結構進行瞭錶徵，併測定瞭其紫外和熒光光譜性質及生物活性。結果錶明：3箇化閤物均具有較彊的熒光和較大的斯託剋斯位移，對雙鏈DNA（AT、GC）和 G-四鏈體DNA（Hum24）均有一定的相互作用；DNA的加入能使3箇化閤物的熒光髮生猝滅，其中熒光猝滅程度與雙鏈DNA（AT、GC）的濃度呈線性關繫。據此可將這3箇化閤物開髮用作熒光猝滅探針以檢測雙鏈DNA（AT、GC）的濃度。
합성료3개신적2-（4-간분기）미서병[4，5-f]1，10-린비라람연생물，통과홍외광보、질보、1 HNMR、원소분석등대기결구진행료표정，병측정료기자외화형광광보성질급생물활성。결과표명：3개화합물균구유교강적형광화교대적사탁극사위이，대쌍련DNA（AT、GC）화 G-사련체DNA（Hum24）균유일정적상호작용；DNA적가입능사3개화합물적형광발생졸멸，기중형광졸멸정도여쌍련DNA（AT、GC）적농도정선성관계。거차가장저3개화합물개발용작형광졸멸탐침이검측쌍련DNA（AT、GC）적농도。
Three novel 2-(4-hydroxyphenyl)imidazo[4,5-f]1,10-phenanthroline derivatives were synthe-sized and their structures were confirmed by IR,MS,1 HNMR and elemental analysis.The UV and fluorescence spectral properties and bioactivities of three compounds were studied.The results showed that three compounds had strong fluorescence with large Stokes shift and could interact with the double-stranded DNA(AT,GC)and the G-quadruplex DNA(Hum24).The fluorescence quenching were detected when the DNAs had been added in-to the solution of three compounds.The fluorescence quenching intensity was linear with the concentration of double-stranded DNA(AT,GC).So,three compounds could be used to detect the concentration of double-stran-ded DNA(AT,GC)as fluorescence quenching probe.
