CAJ | 학술논문

中药通光散（ Marsdenia tenacissima）用于肿瘤治疗，研究其抗肿瘤或肿瘤化疗增敏活性提取物具有重要意义。研究发现，浓度为10μg/mL的通光散总苷对白血病K562、宫颈癌HeLa、肝癌HepG2、口腔上皮癌KB-3-14种人类肿瘤细胞的生长无影响，细胞活力均达到98％以上，以此为工作浓度联用紫杉醇处理肿瘤细胞，发现与单用紫杉醇组相比，联用后能够显著增强紫杉醇的体外抗肿瘤作用。对活性提取物开展的分析方法研究中，选择6种通光散苷元乙衍生物单体（化合物1~6）作为专属性对照品，采用Phenomenex Luna C18色谱柱，在230 nm下以甲醇-0.1％乙酸水进行梯度洗脱，获得了对通光散总苷中25个成分的良好色谱分离，以及对6种专属性指标成分的辨识；在色谱图中指标成分1~6的色谱峰面积之和为可检测成分总峰面积的43.39％。同时建立了通光散总苷中专属性指标成分1的含量测定方法。所得方法的峰面积精度、含量测定重现性、样品稳定性等数据的相对标准偏差（ RSD）为0.16％~0.55％，样品测定的线性范围为0.86~6.45μg，方法回收率为100.4％±1.2％；测得指标成分1的含量为0.74％。
중약통광산（ Marsdenia tenacissima）용우종류치료，연구기항종류혹종류화료증민활성제취물구유중요의의。연구발현，농도위10μg/mL적통광산총감대백혈병K562、궁경암HeLa、간암HepG2、구강상피암KB-3-14충인류종류세포적생장무영향，세포활력균체도98％이상，이차위공작농도련용자삼순처리종류세포，발현여단용자삼순조상비，련용후능구현저증강자삼순적체외항종류작용。대활성제취물개전적분석방법연구중，선택6충통광산감원을연생물단체（화합물1~6）작위전속성대조품，채용Phenomenex Luna C18색보주，재230 nm하이갑순-0.1％을산수진행제도세탈，획득료대통광산총감중25개성분적량호색보분리，이급대6충전속성지표성분적변식；재색보도중지표성분1~6적색보봉면적지화위가검측성분총봉면적적43.39％。동시건립료통광산총감중전속성지표성분1적함량측정방법。소득방법적봉면적정도、함량측정중현성、양품은정성등수거적상대표준편차（ RSD）위0.16％~0.55％，양품측정적선성범위위0.86~6.45μg，방법회수솔위100.4％±1.2％；측득지표성분1적함량위0.74％。
Chinese materia medica Tong-Guang-San( Marsdenia tenacissima)is used for treatment of cancer. Developing a bioactive fraction from this plant with optimized components as antitumor agent or chemosensitizer is of great importance. This paper reports results of methodological research for quality control of extract of total glycosides( ETG ), a chemosensitive fraction screened out. ln our study,ETG at the concentration of 10μg/mL did not affect the growth of human leukemia cell line K562, human cervical carcinoma cell line HeLa,human hepatoma cell line HepG2 and human oral epidermoid carcinoma cell line KB-3-1( Cell viability>98%),but when 10 μg/mL ETG was used in combination with paclitaxel,the cytoxicity of paclitaxel against above tumor cells was significantly increased. By em-ploying a Phenomenex Luna C18 column as stationary phase,methanol-0. 1% HAc as mobile phase for gradient elution,and 6 ester derivatives of tenacigenin B(1-6)as reference markers,an HPLC method was used for the qualitative analysis of ETG,with well separation of 25 components and identification of the 6 structurally characteristic markers. The summed peak area for 1-6 accounted for 43. 39% of the to-tal peak area. The quantitative analysis of reference 1 was also completed,with relative standard devia-tions( RSD ) for peak area, repeatability of measured concentration and stability of sample within 0. 16% -0. 55%,linear range of 0. 86-6. 45 μg,and recoveries of 100. 4% ± 1. 2%. The method was applied in the determination of total glycosides of Marsdenia tenacissima with reference 1 amount of 0. 74%.