分析测试学报
分析測試學報
분석측시학보
JOURNAL OF INSTRUMENTAL ANALYSIS
2014年
10期
1194-1198,1203
,共6页
彭池方%谢正军%汪雅云%宋珊珊
彭池方%謝正軍%汪雅雲%宋珊珊
팽지방%사정군%왕아운%송산산
纳米金%纳米复合薄膜%铅离子%肉眼检测
納米金%納米複閤薄膜%鉛離子%肉眼檢測
납미금%납미복합박막%연리자%육안검측
gold nanoparticle%nanocomposite film%lead ions%naked eye detection
基于2-巯基乙醇-硫代硫酸钠-纳米金/Pb2＋（2-ME-Na2 S2 O3-AuNPs/Pb2＋）体系的纳米金浸出反应，开发了一种低成本、可通过肉眼灵敏检测Pb2＋的纳米复合薄膜。优选出聚酰胺-6（ PA-6）层析薄膜，通过吸附牛血清白蛋白（ BSA）修饰的纳米金（ AuNPs），制备得到一种色泽均匀的BSA-AuNPs/PA-6纳米复合薄膜。考察了 PA-6薄膜吸附 BSA - AuNPs 的时间，2-ME - Na2 S2 O3- AuNPs/Pb2＋反应体系中2-ME 和Na2 S2 O3的浓度，以及反应温度和反应时间对Pb2＋检测的影响。结果表明，在优化条件下，吸附30 min后，0.05 mol/L的Na2 S2 O3及0.25 mol/L的2-ME在80℃下反应20 min，即可通过肉眼实现对Pb2＋的检测，灵敏度可达2.0×10-8 mol/L；同时，该方法具有高选择性。将BSA-AuNPs/ PA-6纳米复合薄膜应用于自来水中Pb2＋的检测，通过肉眼判断，检出限可达到5.0×10-8 mol/L。
基于2-巰基乙醇-硫代硫痠鈉-納米金/Pb2＋（2-ME-Na2 S2 O3-AuNPs/Pb2＋）體繫的納米金浸齣反應，開髮瞭一種低成本、可通過肉眼靈敏檢測Pb2＋的納米複閤薄膜。優選齣聚酰胺-6（ PA-6）層析薄膜，通過吸附牛血清白蛋白（ BSA）脩飾的納米金（ AuNPs），製備得到一種色澤均勻的BSA-AuNPs/PA-6納米複閤薄膜。攷察瞭 PA-6薄膜吸附 BSA - AuNPs 的時間，2-ME - Na2 S2 O3- AuNPs/Pb2＋反應體繫中2-ME 和Na2 S2 O3的濃度，以及反應溫度和反應時間對Pb2＋檢測的影響。結果錶明，在優化條件下，吸附30 min後，0.05 mol/L的Na2 S2 O3及0.25 mol/L的2-ME在80℃下反應20 min，即可通過肉眼實現對Pb2＋的檢測，靈敏度可達2.0×10-8 mol/L；同時，該方法具有高選擇性。將BSA-AuNPs/ PA-6納米複閤薄膜應用于自來水中Pb2＋的檢測，通過肉眼判斷，檢齣限可達到5.0×10-8 mol/L。
기우2-구기을순-류대류산납-납미금/Pb2＋（2-ME-Na2 S2 O3-AuNPs/Pb2＋）체계적납미금침출반응，개발료일충저성본、가통과육안령민검측Pb2＋적납미복합박막。우선출취선알-6（ PA-6）층석박막，통과흡부우혈청백단백（ BSA）수식적납미금（ AuNPs），제비득도일충색택균균적BSA-AuNPs/PA-6납미복합박막。고찰료 PA-6박막흡부 BSA - AuNPs 적시간，2-ME - Na2 S2 O3- AuNPs/Pb2＋반응체계중2-ME 화Na2 S2 O3적농도，이급반응온도화반응시간대Pb2＋검측적영향。결과표명，재우화조건하，흡부30 min후，0.05 mol/L적Na2 S2 O3급0.25 mol/L적2-ME재80℃하반응20 min，즉가통과육안실현대Pb2＋적검측，령민도가체2.0×10-8 mol/L；동시，해방법구유고선택성。장BSA-AuNPs/ PA-6납미복합박막응용우자래수중Pb2＋적검측，통과육안판단，검출한가체도5.0×10-8 mol/L。
A simple and low-cost composite film was prepared,based on which a sensitive detection method of lead ions(Pb2+)in aqueous solutions with naked eye was developed. Polyamide-6(PA-6) thin layer chromatographic membrane was selected to adsorb bovine serum albumin( BSA)modified gold nanoparticle( AuNPs),and a stable BSA-AuNPs/PA-6 nanocomposite film with a uniform red color was prepared. The adsorption time of BSA - AuNPs to PA-6 membrane, concentrations of Na2 S2 O3 and 2-ME as well as reaction temperature and time in the 2-ME-Na2 S2 O3 -AuNPs/Pb2+leaching system were investigated,which demonstrated obvious effects on the detection of Pb2+. Un-der the optimal conditions( BSA - AuNPs and PA-6 incubate together for 30 min,0. 05 mol/L Na2 S2 O3 ,0. 25 mol/L 2-ME,nanocomposite film and leaching agents incubate together at 80 ℃ for 20 min),the BSA-AuNPs/PA-6 nanocomposite film allowed for the sensitive detection of Pb2+ with naked eye with a sensitivity as low as 2. 0 × 10 -8 mol/L. The method showed a high selectivity,and was applied in the naked eye detection of Pb2+ in tap water with a detection limit of 5. 0 × 10-8 mol/L.
