CAJ | 학술논문

采用胶束毛细管电泳（ MEKC），建立了四环素（ TCs）和青霉素（ PENs）两类7种药物同时分离的方法。考察了MEKC中缓冲液类型、离子浓度和pH值，以及表面活性剂（ SDS）浓度、分离电压、温度等参数的影响。利用L16（45）正交试验，确立了最佳的电泳条件：缓冲液为40 mmol/L 磷酸二氢钾-20 mmol/L 硼砂，添加65 mmol/L SDS，pH 7.9，分离电压28 kV，分离温度28℃，紫外检测波长分别为350 nm 和200 nm。结果表明：7种药物在25 min内得到完全分离。在1.56~50 mg/L 范围内呈良好的线性关系，相关系数（r2）为0.9979~0.9999，峰面积的相对标准偏差（n=6）为4.1％~7.3％；迁移时间的相对标准偏差（n=6）为0.33％~0.67％。在2.0，5.0，10.0 mg/kg的加标水平下，7种药物的回收率为83.6％~93.3％，相对标准偏差（ n=6）为4.7％~7.6％。该法快速、简便、准确，具有较高的灵敏度，已应用于合肥市及周边地区水塘和湖水中7种药物的快速分离检测。
채용효속모세관전영（ MEKC），건립료사배소（ TCs）화청매소（ PENs）량류7충약물동시분리적방법。고찰료MEKC중완충액류형、리자농도화pH치，이급표면활성제（ SDS）농도、분리전압、온도등삼수적영향。이용L16（45）정교시험，학립료최가적전영조건：완충액위40 mmol/L 린산이경갑-20 mmol/L 붕사，첨가65 mmol/L SDS，pH 7.9，분리전압28 kV，분리온도28℃，자외검측파장분별위350 nm 화200 nm。결과표명：7충약물재25 min내득도완전분리。재1.56~50 mg/L 범위내정량호적선성관계，상관계수（r2）위0.9979~0.9999，봉면적적상대표준편차（n=6）위4.1％~7.3％；천이시간적상대표준편차（n=6）위0.33％~0.67％。재2.0，5.0，10.0 mg/kg적가표수평하，7충약물적회수솔위83.6％~93.3％，상대표준편차（ n=6）위4.7％~7.6％。해법쾌속、간편、준학，구유교고적령민도，이응용우합비시급주변지구수당화호수중7충약물적쾌속분리검측。
A method for the simultaneous separation of seven kinds of drugs including tetracyclines (TCs)and penicillins(PENs)by micelle capillary electrophoresis(MEKC)was developed. Effects of some important factors such as composition,pH value and concentration of running buffer,surfac-tant concentration,separation voltage and separation temperature were investigated,and the factors were optimized by the L16(45 )orthogonal test. The optimal conditions were as follows:the running buffer:40 mmol/L potassium dihydrogen phosphate -20 mmol/L borax solution( pH 7. 9 ), SDS concentration:65 mmol/L,separation voltage:28 kV,separation temperature:28 ℃,UV detec-tion wavelength:350 nm and 200 nm. Seven kinds of drugs were baseline separated within 25 min. The calibration curves showed good linearities in the range of 1. 56-50 mg/L,with correlation coef-ficients(r2)of 0. 997 9-0. 999 9. RSDs(n=6)were in the range of 4. 1% -7. 3% for peak area and 0. 33% -0. 67% for migration time. The average recoveries of seven kinds drugs at three spiked concentration levels of 2. 0,5. 0,10. 0 mg/kg ranged from 83. 6% to 93. 3% ,with RSDs(n=6)of 4. 7% -7. 6% . The method was rapid,simple,accurate and high sensitive,and was successfully applied in the determination of seven kinds of drugs in the ponds and lakes of Hefei and its surround-ing areas.