CAJ | 학술논문

为鉴定广东水松的遗传多样性，应用改良 CTAB 法分别提取广东不同地区的孑遗植物水松基因组 DNA，以真核生物 rDNA ITS 序列的通用引物分别对其 ITS 区进行 PCR 扩增及测序后进行比对、分析，将得到的序列申请登录入 GenBank 核苷酸数据库。结果表明：广东地区不同来源水松的 rDNA ITS 序列长度均为988 bp，相互之间仅有2～3个碱基差异，说明该序列可作为从分子水平鉴别水松种质资源的参考标记。珠海、中山和广州地区孑遗植物水松 GenBank 核苷酸数据库登录号分别是 KF977874、KF977876和 KF977875。
위감정엄동수송적유전다양성，응용개량 CTAB 법분별제취엄동불동지구적혈유식물수송기인조 DNA，이진핵생물 rDNA ITS 서렬적통용인물분별대기 ITS 구진행 PCR 확증급측서후진행비대、분석，장득도적서렬신청등록입 GenBank 핵감산수거고。결과표명：엄동지구불동래원수송적 rDNA ITS 서렬장도균위988 bp，상호지간부유2～3개감기차이，설명해서렬가작위종분자수평감별수송충질자원적삼고표기。주해、중산화엄주지구혈유식물수송 GenBank 핵감산수거고등록호분별시 KF977874、KF977876화 KF977875。
The genomic DNA of relic plant G.pensilis from different regions in Guangdong was extracted by the modified CTAB method,then the ITS of genomic DNA was amplified and sequenced by the universal primer of eucaryon rDNA ITS sequence respectively,and finally the obtained sequence by comparison and analysis was applied for entering GenBank.The results showed that the rDNA ITS sequence length of G.pensilis samples from different regions in Guandong is 988 bp and there is difference with 2-3 bases in sequence length between different G.pensilis samples,which means that the sequence can be used as the reference marker to identify Glyptostrobus pensilis germplasm resources based on molecular level. The accession number of G. pensilis from Zhuhai, Zhongshan and Guanzhou is KF977874,KF977876 and KF977875 in GenBank respectively.