CAJ | 학술논문

为筛选薏苡种仁内生真菌的抗菌活性，采用组织分离法，从禾本科黍亚科蜀黍族薏苡种仁中分离内生真菌，采用菌饼法筛选其抗菌活性。通过5．8 S ITS 分子系统学鉴定菌种，高效液相色谱法（HPLC）分析鉴定菌种发酵液正丁醇提取物次的生代谢产物。结果表明：分离共获得23株薏苡种仁内生真菌，其中，有弯孢霉属、链格孢属、砖孢属、茎点霉属、灰软盘菌属、稻黑孢属、玉蜀黍赤霉属、曲霉属及其他真菌。57％的菌株至少对测试菌株中的1种具有抗菌活性，且 GZR19号菌株具有广谱抗菌活性并鉴定为1种茎点霉（Phoma sp ．），其发酵液的次生代谢产物在该色谱条件下主要有4种物质含量较高，其中可能存在具有抗菌活性的化合物。
위사선의이충인내생진균적항균활성，채용조직분리법，종화본과서아과촉서족의이충인중분리내생진균，채용균병법사선기항균활성。통과5．8 S ITS 분자계통학감정균충，고효액상색보법（HPLC）분석감정균충발효액정정순제취물차적생대사산물。결과표명：분리공획득23주의이충인내생진균，기중，유만포매속、련격포속、전포속、경점매속、회연반균속、도흑포속、옥촉서적매속、곡매속급기타진균。57％적균주지소대측시균주중적1충구유항균활성，차 GZR19호균주구유엄보항균활성병감정위1충경점매（Phoma sp ．），기발효액적차생대사산물재해색보조건하주요유4충물질함량교고，기중가능존재구유항균활성적화합물。
The antimicrobial activity of endophytic fungi isolated from Coix lacryma-jobi kernels was screened by the colony method,then the strains were identified by 5.8 S ITS molecular systematics,and finally secondary metabolites extracted from strain fermentation liquor were analyzed by HPLC.The results showed that 23 endophytic fungi strains including Curvularia, Alternaria, Dictyo-mitosporicfungi ,Phoma sp .,Mollisia cinerea ,Nigrospora oryzae ,Gibberella zeae ,Aspergillus and other fungi are isolated.57% strains has the antimicrobial activity against one test strain at least.GZR19 strain with broad-spectrum antimicrobial activity is identified as Phoma sp.and there are main four substances in secondary metabolites of the fermentation liquor,which indicates that the compounds with antimicrobial activity may exist in four substances.