CAJ | 학술논문

为了明确尾孢属真菌的生物学特性，采用菌丝生长速率法及液体振荡培养法，对影响玉簪尾孢叶斑病菌菌株 Ch10菌丝生长的条件进行了研究。结果表明，除光照条件外，其他培养条件对 Ch10菌丝生长均有显著影响；Ch10菌丝生长最适温度为25℃，最适 pH 为6，对乳糖、麦芽糖、酵母膏和硝酸钾利用最好，在 MEA 培养基上的生长略快于其他培养基，但培养21 d 最大菌落直径仅2．37 cm，生长非常缓慢，而且极少产孢。
위료명학미포속진균적생물학특성，채용균사생장속솔법급액체진탕배양법，대영향옥잠미포협반병균균주 Ch10균사생장적조건진행료연구。결과표명，제광조조건외，기타배양조건대 Ch10균사생장균유현저영향；Ch10균사생장최괄온도위25℃，최괄 pH 위6，대유당、맥아당、효모고화초산갑이용최호，재 MEA 배양기상적생장략쾌우기타배양기，단배양21 d 최대균락직경부2．37 cm，생장비상완만，이차겁소산포。
The mycelial growth condition of Ch10 strain,a pathogenic fungus of leaf spot disease in H .plantaginea,was analyzed by mycelial growth rate and liquid shaking culture methods to study the biological characteristics of Cercospora fungus.The results showed that temperature,pH,medium, different carbon and nitrogen sources have the significant effect on mycelial growth of Ch10 strain,and the optimum temperature,pH,carbon and nitrogen sources are 25℃,6,lactose,maltose,yeast extract and potassium nitrate.The mycelial growth of Ch10 strain cultured on MEA medium is slightly faster than other media but its maximum colony diameter is only 2.37cm after 21d culture,and the mycelium forms conidia rarely.