风湿病与关节炎
風濕病與關節炎
풍습병여관절염
Rheumatism and Arthritis
2014年
10期
10-12
,共3页
刘又文%谭旭仪%高书图%陈献韬%贾宇东%孙瑞波
劉又文%譚旭儀%高書圖%陳獻韜%賈宇東%孫瑞波
류우문%담욱의%고서도%진헌도%가우동%손서파
当归补血汤%气滞血瘀证%一氧化氮%血管内皮素-1%深静脉血栓%大鼠
噹歸補血湯%氣滯血瘀證%一氧化氮%血管內皮素-1%深靜脈血栓%大鼠
당귀보혈탕%기체혈어증%일양화담%혈관내피소-1%심정맥혈전%대서
Danggui Buxue Decoction%qi deficiency and blood stagnation syndrome%nitric oxide%endothelin-1%deep vein thrombosis%rat
目的：观察当归补血汤对气虚血瘀证模型大鼠一氧化氮（NO）、血管内皮素（ET）-1的影响。方法：选取清洁级SD大鼠60只，随机分为正常对照组、模型对照组和当归补血汤组，每组20只。在成功建立大鼠气滞血瘀证模型后，当归补血汤组给予当归补血汤灌胃，其余两组给予同剂量生理盐水灌胃，观察3组灌胃后第3天、第7天大鼠NO、ET-1含量，并进行比较。结果：与正常对照组比较，模型对照组与当归补血汤组第3天、第7天时血清NO含量降低，ET-1含量升高，差异有统计学意义（P<0.01）。与模型对照组比较，当归补血汤组在第3天、第7天时血清NO含量上升，ET-1含量下降，差异有统计学意义（P<0.01）。同时，正常对照组和模型对照组血清NO、ET-1含量在第3天、第7天组内比较，差异无统计学意义（P>0.05）。当归补血汤组中第3天与第7天比较，差异有统计学意义（P<0.01）。结论：当归补血汤可有效增加气虚血瘀证模型大鼠NO含量，减少ET-1含量，这可能是其防治深静脉血栓的作用机制。
目的：觀察噹歸補血湯對氣虛血瘀證模型大鼠一氧化氮（NO）、血管內皮素（ET）-1的影響。方法：選取清潔級SD大鼠60隻，隨機分為正常對照組、模型對照組和噹歸補血湯組，每組20隻。在成功建立大鼠氣滯血瘀證模型後，噹歸補血湯組給予噹歸補血湯灌胃，其餘兩組給予同劑量生理鹽水灌胃，觀察3組灌胃後第3天、第7天大鼠NO、ET-1含量，併進行比較。結果：與正常對照組比較，模型對照組與噹歸補血湯組第3天、第7天時血清NO含量降低，ET-1含量升高，差異有統計學意義（P<0.01）。與模型對照組比較，噹歸補血湯組在第3天、第7天時血清NO含量上升，ET-1含量下降，差異有統計學意義（P<0.01）。同時，正常對照組和模型對照組血清NO、ET-1含量在第3天、第7天組內比較，差異無統計學意義（P>0.05）。噹歸補血湯組中第3天與第7天比較，差異有統計學意義（P<0.01）。結論：噹歸補血湯可有效增加氣虛血瘀證模型大鼠NO含量，減少ET-1含量，這可能是其防治深靜脈血栓的作用機製。
목적：관찰당귀보혈탕대기허혈어증모형대서일양화담（NO）、혈관내피소（ET）-1적영향。방법：선취청길급SD대서60지，수궤분위정상대조조、모형대조조화당귀보혈탕조，매조20지。재성공건립대서기체혈어증모형후，당귀보혈탕조급여당귀보혈탕관위，기여량조급여동제량생리염수관위，관찰3조관위후제3천、제7천대서NO、ET-1함량，병진행비교。결과：여정상대조조비교，모형대조조여당귀보혈탕조제3천、제7천시혈청NO함량강저，ET-1함량승고，차이유통계학의의（P<0.01）。여모형대조조비교，당귀보혈탕조재제3천、제7천시혈청NO함량상승，ET-1함량하강，차이유통계학의의（P<0.01）。동시，정상대조조화모형대조조혈청NO、ET-1함량재제3천、제7천조내비교，차이무통계학의의（P>0.05）。당귀보혈탕조중제3천여제7천비교，차이유통계학의의（P<0.01）。결론：당귀보혈탕가유효증가기허혈어증모형대서NO함량，감소ET-1함량，저가능시기방치심정맥혈전적작용궤제。
Objective:To observe effects of Danggui Buxue Decoction on Nitric Oxide(NO) and Endothelin-1(ET-1) of model rats with qi deifciency and blood stagnation.Methods:60 SD rats of clean grade were randomly and equally divided into a normal control group,a model control group and a Danggui Buxue Decoction group.After the model rats with qi deifciency and blood stagnation were established,the Danggui Buxue Decoction group were given intragastric administration with Danggui Buxue Decoction,while the other two group were given intragastric administration with the same dose of saline water.The contents of NO and ET-1 of the three groups were observed and compared on the third and seventh days after lavage.Results:Compared with the normal control group,the contents of NO of the other two groups decreased and their ET-1 contents increased on the third and seventh days,the difference being statistically signiifcant(P< 0.01).Compared with the model group,the NO content of the Danggui Buxue Decoction group increased on third and seventh days,while the ET-1 content decreased,the difference being statistically signiifcant(P< 0.01).At the same time,the differences of serum NO and ET-1 contents between the normal control group and the model control group on the third and seventh days were not signiifcantly different(P> 0.05).Comparing the contents of NO and ET-1 among the Danggui Buxue Decoction group on the third day and the seventh day,the differences were statistically signiifcant (P< 0.01).Conclusion:Danggui Buxue Decoction can effectively increase the content of NO and decrease the content of ET-1 in the rat model of qi deifciency and blood stagnation,which may be its mechanism of preventing and treating deep venous thrombosis.
