贵州农业科学
貴州農業科學
귀주농업과학
GUIZHOU AGRICULTURAL SCIENCES
2014年
10期
106-108
,共3页
王霞%孙仓%刘小玲%杨祥波
王霞%孫倉%劉小玲%楊祥波
왕하%손창%류소령%양상파
稠李%果实%原花青素%抗氧化活性
稠李%果實%原花青素%抗氧化活性
주리%과실%원화청소%항양화활성
Padus racemosa%fruit%procyanidine%antioxidant activity
为稠李果实的进一步开发利用提供基础依据,采用亚油酸体系-硫氰酸铁测定原花青素抗氧化力等方法,研究稠李果实原花青素对 DPPH·、O2-·和·OH 3种自由基的清除效果,并与抗坏血酸的抗氧化力进行比较。结果表明:0.15 mg/mL 的稠李果实原花青素和抗坏血酸均能不同程度抑制亚油酸自氧化,抑制率分别为46.9%和16.9%;稠李果实原花青素对 DPPH·、·OH 和 O2-·的最大清除率分别为97.8%、32.0%和96.5%,抗坏血酸对 DPPH·、· OH 和 O2-·的最大清除率分别为43.0%、27.4%和75.5%。稠李果实原花青素和抗坏血酸对自由基的清除率均随浓度增加而呈上升趋势,稠李果实原花青素的抗氧化性明显强于抗坏血酸。
為稠李果實的進一步開髮利用提供基礎依據,採用亞油痠體繫-硫氰痠鐵測定原花青素抗氧化力等方法,研究稠李果實原花青素對 DPPH·、O2-·和·OH 3種自由基的清除效果,併與抗壞血痠的抗氧化力進行比較。結果錶明:0.15 mg/mL 的稠李果實原花青素和抗壞血痠均能不同程度抑製亞油痠自氧化,抑製率分彆為46.9%和16.9%;稠李果實原花青素對 DPPH·、·OH 和 O2-·的最大清除率分彆為97.8%、32.0%和96.5%,抗壞血痠對 DPPH·、· OH 和 O2-·的最大清除率分彆為43.0%、27.4%和75.5%。稠李果實原花青素和抗壞血痠對自由基的清除率均隨濃度增加而呈上升趨勢,稠李果實原花青素的抗氧化性明顯彊于抗壞血痠。
위주리과실적진일보개발이용제공기출의거,채용아유산체계-류청산철측정원화청소항양화력등방법,연구주리과실원화청소대 DPPH·、O2-·화·OH 3충자유기적청제효과,병여항배혈산적항양화력진행비교。결과표명:0.15 mg/mL 적주리과실원화청소화항배혈산균능불동정도억제아유산자양화,억제솔분별위46.9%화16.9%;주리과실원화청소대 DPPH·、·OH 화 O2-·적최대청제솔분별위97.8%、32.0%화96.5%,항배혈산대 DPPH·、· OH 화 O2-·적최대청제솔분별위43.0%、27.4%화75.5%。주리과실원화청소화항배혈산대자유기적청제솔균수농도증가이정상승추세,주리과실원화청소적항양화성명현강우항배혈산。
The scavenging effect of procyanidine in Padus racemosa fruits on DPPH ·,O2- · and ·OH free radicals was studied by the linoleic acid-thiocyanatoiron method,and the antioxidant resistance was compared with ascorbic acid to provide the scientific basis for further development and utilization of Padus Racemosa fruits. The results showed that 0.15 mg/mL procyanidine extracted from Padus racemosa fruits and ascorbic acid both can inhibit auto-oxidation of linoleic acid to varying degrees,and the inhibition rate of procyanidine and ascorbic acid is 46.9% and 16.9% respectively.The maximum scavenging rate of procyanidine extracted from Padus racemosa fruits to DPPH ·,·OH and O2- is 97.8%,32.0% and 96.5% separately,and the maximum scavenging rate of ascorbic acid to DPPH·, ·OH and O2- · is 43.0%,27.4% and 75.5% respectively.The scavenging rate of procyanidine extracted from Padus racemosa fruits and ascorbic acid to free radicals shows a rising trend with increase of concentration,and the inoxidizability of procyanidine extracted from Padus racemosa fruits is higher than ascorbic acid significantly.