CAJ | 학술논문

为了给禾谷炭疽菌新靶标药物的研究开发提供参考，以酿酒酵母的典型腺苷酸环化酶（AC）序列为基础，利用炭疽菌属蛋白数据库在线对其进行 Blastp 比对及关键词搜索，通过 SMART 对该菌典型AC 蛋白的保守结构域、理化性质、细胞信号肽、跨膜区结构以及二级结构等生物信息学进行分析，同时比较禾谷炭疽菌中典型 AC 氨基酸序列与其他物种同源序列的遗传关系。结果表明：禾谷炭疽菌中存在一个与酿酒酵母 Srv2同源的序列，命名为 CgCap1腺苷酸环化酶蛋白，该酶蛋白包含有530个氨基酸，基因序列长1906 bp 具有保守 CARP 结构域，等电点蛋白在5～6范围，属亲水性、不稳定蛋白，不含信号肽序列，定位于质膜上，二级结构中的β折叠所占比例较低，与同属于炭疽菌属真菌胶孢炭疽菌、西瓜炭疽菌的亲缘关系较近。
위료급화곡탄저균신파표약물적연구개발제공삼고，이양주효모적전형선감산배화매（AC）서렬위기출，이용탄저균속단백수거고재선대기진행 Blastp 비대급관건사수색，통과 SMART 대해균전형AC 단백적보수결구역、이화성질、세포신호태、과막구결구이급이급결구등생물신식학진행분석，동시비교화곡탄저균중전형 AC 안기산서렬여기타물충동원서렬적유전관계。결과표명：화곡탄저균중존재일개여양주효모 Srv2동원적서렬，명명위 CgCap1선감산배화매단백，해매단백포함유530개안기산，기인서렬장1906 bp 구유보수 CARP 결구역，등전점단백재5～6범위，속친수성、불은정단백，불함신호태서렬，정위우질막상，이급결구중적β절첩소점비례교저，여동속우탄저균속진균효포탄저균、서과탄저균적친연관계교근。
The typical AC sequence was compared with on-line C.protein databank by Blastp comparison,then the bioinformatics of conserved domain,physicochemical property,cell signal peptide, structure of transmembrane domain and secondary structure was analyzed by SMART and the genetic relationships between typical AC sequence in C.graminicola and the homologous sequence of other species were compared to provide a reference for development and utilization of new medicines with drug target of C.graminicola.The results showed that CgCap1in C.graminicola is a homologous protein with Srv2 in S. cerevisiae.CgCap 1 located in the plasma membrane with conserved CARP domain and isoeletric point with 5~6 range belongs to the sequence of hydrophily,instable protein and without signal peptide.The proportion of βfold is low in the secondary structure.The genetic relationship between CgCap 1 and Colletoreichum is close.