CAJ | 학술논문

目的：观察“标本配穴”针刺对胰岛素抵抗大鼠股四头肌中三酰甘油(TG)含量的影响,探讨针刺提高胰岛素敏感性的机制。方法8周龄Wistar大鼠32只,随机分为正常组、模型组、电针组、假电针组,每组8只。正常组予基础饲料,其余各组予高脂饲料喂养,8周后形成胰岛素抵抗模型,电针组予“标本配穴”针刺法,取“关元”、“中脘”及双侧“足三里”、“丰隆”电针治疗8周,假电针组大鼠予电针组穴位附近、穴位所在经络外非穴区电针治疗。记录各组大鼠进食量和体质量,应用血糖仪检测各组血糖,酶法测定股四头肌中TG含量。结果与模型组比较,电针组、假电针组大鼠体质量和股四头肌中TG含量均下降,尤以电针组下降明显,差异有统计学意义(P＜0.05)。结论“标本配穴”针刺可以预防胰岛素抵抗,提高胰岛素敏感性。
목적：관찰“표본배혈”침자대이도소저항대서고사두기중삼선감유(TG)함량적영향,탐토침자제고이도소민감성적궤제。방법8주령Wistar대서32지,수궤분위정상조、모형조、전침조、가전침조,매조8지。정상조여기출사료,기여각조여고지사료위양,8주후형성이도소저항모형,전침조여“표본배혈”침자법,취“관원”、“중완”급쌍측“족삼리”、“봉륭”전침치료8주,가전침조대서여전침조혈위부근、혈위소재경락외비혈구전침치료。기록각조대서진식량화체질량,응용혈당의검측각조혈당,매법측정고사두기중TG함량。결과여모형조비교,전침조、가전침조대서체질량화고사두기중TG함량균하강,우이전침조하강명현,차이유통계학의의(P＜0.05)。결론“표본배혈”침자가이예방이도소저항,제고이도소민감성。
Objective To observe the effects of acupuncture therapy on triglyceride (TG) content of quadriceps femoris in the high fat diet feeding-induced insulin resistance rats; To discuss acupuncture mechanisms to improve the insulin sensitivity. Methods Thirty-two Wistar rats at the age of eight weeks were randomly divided into normal group, model group, electro-acupuncture group, and sham electro-acupuncture group. The normal group was given basic diet, and model group, electro-acupuncture group and sham electro-acupuncture group were given high fat diet for 8 weeks, after which, the model of insulin resistance was successfully obtained. Electro-acupuncture group was given acupuncture therapy with “Guanyuan (GV4)”, “Zhognwan (GV12)”, “Zusanli (ST36)”, and “Fenglong (ST40)” for 8 weeks. Sham electro-acupuncture group was given stimulation at non-acupuncture points which were not on the meridians and near the acupoints but not at the area of the acupoints. In addition, food intake and body weight of each group were recorded, blood glucose was tested, TG content of quadriceps femoris was measured by enzymatic method after the treatment. Results The weight of the rats and TG levels of quadriceps femoris in electro-acupuncture group and sham electro-acupuncture group were significantly lower than those in model group, with significant statistical difference (P<0.05), especially in electro-acupuncture group. Conclusion Acupuncture therapy could improve insulin sensitivity to prevent and its related diseases.