CAJ | 학술논문

目的：观察电针对慢性应激抑郁模型大鼠海马E-选择素和抵抗素蛋白表达的影响,探讨电针对抑郁症脑内微环境的作用机制。方法采用慢性应激结合孤养方法造模。将40只SD大鼠随机分为空白组、模型组、电针组、氟西汀组,每组10只。于每日造模前1 h,电针干预“百会”、“印堂”,频率2 Hz,电流强度0.6 mA,每次20 min；氟西汀灌胃干预,用药剂量10 mg/kg,给药容积5 mL/kg。采用生物素标记抗体芯片技术检测大鼠海马E-选择素和抵抗素蛋白表达水平。结果与空白组比较,模型组大鼠海马E-选择素和抵抗素蛋白水平上调(fold change＝1.23,1.22)；与模型组比较,电针组、氟西汀组 E-选择素水平下调(fold change＝0.65,0.62)、抵抗素表达水平下调(fold change＝0.76,0.65)。结论电针干预可下调慢性应激抑郁模型大鼠海马E-选择素和抵抗素蛋白表达。
목적：관찰전침대만성응격억욱모형대서해마E-선택소화저항소단백표체적영향,탐토전침대억욱증뇌내미배경적작용궤제。방법채용만성응격결합고양방법조모。장40지SD대서수궤분위공백조、모형조、전침조、불서정조,매조10지。우매일조모전1 h,전침간예“백회”、“인당”,빈솔2 Hz,전류강도0.6 mA,매차20 min；불서정관위간예,용약제량10 mg/kg,급약용적5 mL/kg。채용생물소표기항체심편기술검측대서해마E-선택소화저항소단백표체수평。결과여공백조비교,모형조대서해마E-선택소화저항소단백수평상조(fold change＝1.23,1.22)；여모형조비교,전침조、불서정조 E-선택소수평하조(fold change＝0.65,0.62)、저항소표체수평하조(fold change＝0.76,0.65)。결론전침간예가하조만성응격억욱모형대서해마E-선택소화저항소단백표체。
Objective To observe the effects of electro-acupuncture on the E-selectin and resistin protein expression in the hippocampus of rats of chronic stress depression;To explore the role and mechanism of electro-acupuncture in the micro-environment of brain with depression. Methods 40 Fourty rats were randomly divided into four groups:blank group, model group, model+EA group, and model+fluoxetine group by using chronic stress combined with solitary raising methods, 10 rats in each group. Electro-acupuncture intervention was used 1 hour before the modeling. Electro-acupuncture was given at points Baihui (Du 20) and Yintang (Extra) for twenty minutes (2 Hz, 0.6 mA) every day. Fluoxetine was given at the volume of 5 mL/kg, dosage of 10 mg/kg. E-selectin and resistin protein expression in hippocampus were determined by biotin label-based antibody array. Results Compared with the blank group, the protein expression of E-selectin and resistin in the model group increased (fold change=1.23, 1.22). Compared with the model group, E-selectin and resistin expression decreased (fold change=0.65, 0.62;fold change=0.76, 0.65). Conclusion Electro-acupuncture intervention could down-regulate E-selectin and resistin expression in the hippocampus of chronic stress depression model.