CAJ | 학술논문

目的：观察通阳活血方对模拟缺血再灌注损伤兔窦房结细胞骨架微管蛋白β-tubulin的影响，探讨其治疗病态窦房结综合征的机制。方法取新生乳兔窦房结细胞，以缺氧缺糖模拟缺血，以恢复氧和糖的供应模拟再灌注造成窦房结细胞损伤模型，将细胞分为空白组、模型组和通阳活血方高、中、低剂量组。治疗组给予通阳活血方相应浓度药物，空白组与模型组给予等容积培养基，采用酶标仪、激光共聚焦显微镜观察各组窦房结细胞活力、细胞骨架微管蛋白β-tubulin 形态变化。结果模型组存活细胞量较空白组明显减少(P＜0.01)；β-tubulin裂解明显。通阳活血方高、中、低剂量组存活细胞量明显高于模型组(P＜0.05)，β-tubulin结构较模型组明显完整。结论通阳活血方可抑制模拟缺血再灌注引起的兔窦房结细胞损伤，其治疗病态窦房结综合征的机制可能与保护窦房结细胞活力及细胞骨架微管蛋白β-tubulin形态结构有关。
목적：관찰통양활혈방대모의결혈재관주손상토두방결세포골가미관단백β-tubulin적영향，탐토기치료병태두방결종합정적궤제。방법취신생유토두방결세포，이결양결당모의결혈，이회복양화당적공응모의재관주조성두방결세포손상모형，장세포분위공백조、모형조화통양활혈방고、중、저제량조。치료조급여통양활혈방상응농도약물，공백조여모형조급여등용적배양기，채용매표의、격광공취초현미경관찰각조두방결세포활력、세포골가미관단백β-tubulin 형태변화。결과모형조존활세포량교공백조명현감소(P＜0.01)；β-tubulin렬해명현。통양활혈방고、중、저제량조존활세포량명현고우모형조(P＜0.05)，β-tubulin결구교모형조명현완정。결론통양활혈방가억제모의결혈재관주인기적토두방결세포손상，기치료병태두방결종합정적궤제가능여보호두방결세포활력급세포골가미관단백β-tubulin형태결구유관。
Objective To observe the effects of Tongyang Huoxue Recipe on cytoskeleton proteinβ-tubulin of ischemia reperfusion injured sinoatrial node cell in rabbits;To discuss its mechanisms in the treatment of sick sinus syndrome. Methods Sinoatrial node cells were obtained from newborn rabbit. Oxygen and glucose were deprived to simulate ischemia and were restored to simulate reperfusion. Cells were divided into 5 groups. Tongyang Huoxue Recipe high-, medium-, low dose groups were given corresponding medicine (final concentrations of 100, 20, 10 μg/mL). The normal group and model group were given equal volume of culture medium. Enzyme mark instrument and laser scanning confocal microscopy were used to observe the sinoatrial node cell activity and cytoskeleton protein β-tubulin of each group. Results Living cells of model group decreased significantly compared with normal group (P<0.01), and cytoskeleton proteinβ-tubulin cleavage significantly. Living cells of Tongyang Huoxue Recipe high, medium and low dose group were significantly higher than those in the model group (P<0.05), and β-tubulin structure remained more complete than the model group. Conclusion Tongyang Huoxue Recipe can inhibit the injury induced by ischemia reperfusion of sinoatrial node cells;the possible mechanism of Tongyang Huoxue Recipe in treating sick sinus syndrome is protecting activity and cytoskeleton proteinβ-tubulin of sinoatrial node cells.