湖北民族学院学报（自然科学版）
湖北民族學院學報（自然科學版）
호북민족학원학보（자연과학판）
JOURNAL OF HUBEI INSTITUTE FOR NATIONALITIES（NATURAL SCIENCE EDITION）
2014年
3期
276-281
,共6页
金晶%罗爱民%杨艳%张升晖
金晶%囉愛民%楊豔%張升暉
금정%라애민%양염%장승휘
橘皮%黄酮类化合物%正交试验%提取工艺%自由基%抗氧化
橘皮%黃酮類化閤物%正交試驗%提取工藝%自由基%抗氧化
귤피%황동류화합물%정교시험%제취공예%자유기%항양화
orange peel%flavonoids%orthogonal experimental design%extraction process%free group%an-tioxidation
试验从微波辐射辅助处理后的橘皮中提取黄酮类化合物，确定了最优的实验操作参数，同时研究了黄酮类化合物提取液对羟基自由基（· OH）和超氧阴离子（ O2-·）的清除效果．采用直接回流法，以芦丁为对照品，通过UV2050紫外光谱仪对橘皮中的黄酮类化合物含量进行了测定，并计算出产率．在单因素试验的结果上运用正交试验设计对黄酮类化合物的提取工艺进行了优化，得出橘皮中提取黄酮类化合物最优工艺条件为：时间180 min，温度80℃，物料比1∶10，70％的乙醇，pH＝7．在此工艺条件下黄酮类化合物的提取率最高，其提取率可达86．73 mg/g．此方法较传统提取工艺优良，且条件容易达到和控制．橘皮中黄酮类化合物提取液对羟基自由基（· OH）和超氧阴离子（O2·-）具有一定的清除效果，当黄酮类化合物浓度为0．18 mg/mL时对· OH清除效果高达37．72％，0．14 mg/mL时对O2·-清除效果可达28．49％．
試驗從微波輻射輔助處理後的橘皮中提取黃酮類化閤物，確定瞭最優的實驗操作參數，同時研究瞭黃酮類化閤物提取液對羥基自由基（· OH）和超氧陰離子（ O2-·）的清除效果．採用直接迴流法，以蘆丁為對照品，通過UV2050紫外光譜儀對橘皮中的黃酮類化閤物含量進行瞭測定，併計算齣產率．在單因素試驗的結果上運用正交試驗設計對黃酮類化閤物的提取工藝進行瞭優化，得齣橘皮中提取黃酮類化閤物最優工藝條件為：時間180 min，溫度80℃，物料比1∶10，70％的乙醇，pH＝7．在此工藝條件下黃酮類化閤物的提取率最高，其提取率可達86．73 mg/g．此方法較傳統提取工藝優良，且條件容易達到和控製．橘皮中黃酮類化閤物提取液對羥基自由基（· OH）和超氧陰離子（O2·-）具有一定的清除效果，噹黃酮類化閤物濃度為0．18 mg/mL時對· OH清除效果高達37．72％，0．14 mg/mL時對O2·-清除效果可達28．49％．
시험종미파복사보조처리후적귤피중제취황동류화합물，학정료최우적실험조작삼수，동시연구료황동류화합물제취액대간기자유기（· OH）화초양음리자（ O2-·）적청제효과．채용직접회류법，이호정위대조품，통과UV2050자외광보의대귤피중적황동류화합물함량진행료측정，병계산출산솔．재단인소시험적결과상운용정교시험설계대황동류화합물적제취공예진행료우화，득출귤피중제취황동류화합물최우공예조건위：시간180 min，온도80℃，물료비1∶10，70％적을순，pH＝7．재차공예조건하황동류화합물적제취솔최고，기제취솔가체86．73 mg/g．차방법교전통제취공예우량，차조건용역체도화공제．귤피중황동류화합물제취액대간기자유기（· OH）화초양음리자（O2·-）구유일정적청제효과，당황동류화합물농도위0．18 mg/mL시대· OH청제효과고체37．72％，0．14 mg/mL시대O2·-청제효과가체28．49％．
The flavonoids were extracted from orange peel, which was controlled with assisted microwave radiation.And the optimal operating parameter was obtained.At the same time, the antioxidant activities of flavonoids from orange peel, hydroxyl radicals (· OH) and superoxide anion (O2·- ) were discussed and compared with Vc.The extraction yield of flavonoids from orange peel was determined by the detecting of UV2050 ultraviolet spectrometer and rutin was as the reference substance.Based on the result of single factor experiment,the orthogonal experimental design method was used in order to find the optimal experi-mental parameters.The optimal experiment parameters are as follows:70%( V ∶V) ethanol as the sol-vent,the extraction time of 180 min, the extraction temperature of 80℃,the ratio solid/liquid of 1 ∶10 and pH of 7.Under the optimal condition, the flavonoids could reach as high as 86.73mg/g.The fla-vonoids from orange peel have the scavenging capacity for hydroxyl radical ( · OH) and superoxide anion (O2·- ).When the concentration of flavonoids reached as high as 0.18mg/mL,the scavenging capacity of · OH was 37.72%and the scavenging capacity of O2 ·- was 28.51%when the concentrations of fla-vonoids were 0.14 mg/mL.The free radical scavenging capacity of Vc was better than that of flavonoids.
