CAJ | 학술논문

为了研究黄花蒿青蒿素含量与腺毛状毛状体密度的关系，分别利用体视荧光显微镜和扫描电镜测定了8组青蒿素产量不同的黄花蒿的叶片腺毛状毛状体密度，同时利用HPLC法测定相应叶片黄花蒿素含量，再用SPSS软件对二者相关性进行研究．结果表明：①不同植株黄花蒿的叶片腺毛状毛状体密度不同，体式荧光显微镜和扫描电镜观测的最大值均是植株4号样，所得平均密度分别是（75．7±16．0）个/cm2和（29．4±5．9）个/cm2，说明体视荧光显微镜适合检测黄花蒿腺毛状毛状体密度，而扫描电镜观察腺毛状毛状体结构的效果更好；②黄花蒿叶片腺毛状毛状体密度与青蒿素含量的关系存在显著的线性相关，相关系数0．734，P＝0．038＜0．05．这再次证明了青蒿素主要在叶片的腺毛状毛状体内产生，而相关的显著性未能达到极显著水平，说明也有少量青蒿素在其它类别的细胞内产生．
위료연구황화호청호소함량여선모상모상체밀도적관계，분별이용체시형광현미경화소묘전경측정료8조청호소산량불동적황화호적협편선모상모상체밀도，동시이용HPLC법측정상응협편황화호소함량，재용SPSS연건대이자상관성진행연구．결과표명：①불동식주황화호적협편선모상모상체밀도불동，체식형광현미경화소묘전경관측적최대치균시식주4호양，소득평균밀도분별시（75．7±16．0）개/cm2화（29．4±5．9）개/cm2，설명체시형광현미경괄합검측황화호선모상모상체밀도，이소묘전경관찰선모상모상체결구적효과경호；②황화호협편선모상모상체밀도여청호소함량적관계존재현저적선성상관，상관계수0．734，P＝0．038＜0．05．저재차증명료청호소주요재협편적선모상모상체내산생，이상관적현저성미능체도겁현저수평，설명야유소량청호소재기타유별적세포내산생．
In order to study the content of artemisinin and the density of glandular trichomes,the fluores-cence microscope and scanning electron microscopy ( sem) are used to determinate 8 groups of different artemisinin yield.At the same time,the artemisinin content was determined by HPLC method,then SPSS software is used to study correlation between them.The results show that:①the density of glandular tri-chomes is different for different leaves, the maximum mean value observed through fluorescence micro-scope and scanning electron microscopy (sem) was No.4 plant,the average density was (75.7±16.0)a/cm2 ,respectively,and (29.4±5.9) a/cm2 ,which proves fluorescence microscope is more suitable for the determination of the density of glandular trichemes,and scanning electron microscopy ( sem) is better sui-ted to observe the structure of the glandular trichomes;②there is a significant linear correlation relation-ship the density of glandular trichomes and the content of artemisinin,the correlation index is 0.734,P=0.038<0.05.It proves that artemisinin is mainly producted by glandular trichemes,and the associated sig-nificance does not reach extremely significant level, which means a small amount of artemisinin is pro-duced in other types of cells.