CAJ | 학술논문

获得高质量的DNA通常是开展分子水平研究的关键一步。采用经典CTAB法、改良CTAB法、简易CTAB法、SDS微量法及Zigenhagen法对华山松的茎、叶及胚乳的DNA进行提取，并进行琼脂糖凝胶电泳检测、紫外分光光度计分析和标准差及标准误分析，结果表明：对于华山松针叶DNA提取，改良CTAB法提取DNA浓度适中，标准差和标准误最小，方法稳定性好；对于华山松茎段DNA提取，Zigenhagen法提取DNA浓度高，纯度好，标准差和标准误最小，方法的稳定性最好；对于华山松胚乳DNA提取，简易CTAB法提取的DNA浓度最高，纯度最好，标准差和标准误最小，方法的稳定性最好。这也说明对于同一植物的不同部位DNA的提取要采用不同的方法，需要在实践中加以摸索，获得最大的提取效果。
획득고질량적DNA통상시개전분자수평연구적관건일보。채용경전CTAB법、개량CTAB법、간역CTAB법、SDS미량법급Zigenhagen법대화산송적경、협급배유적DNA진행제취，병진행경지당응효전영검측、자외분광광도계분석화표준차급표준오분석，결과표명：대우화산송침협DNA제취，개량CTAB법제취DNA농도괄중，표준차화표준오최소，방법은정성호；대우화산송경단DNA제취，Zigenhagen법제취DNA농도고，순도호，표준차화표준오최소，방법적은정성최호；대우화산송배유DNA제취，간역CTAB법제취적DNA농도최고，순도최호，표준차화표준오최소，방법적은정성최호。저야설명대우동일식물적불동부위DNA적제취요채용불동적방법，수요재실천중가이모색，획득최대적제취효과。
It is the critical first step how to obtain high-quality DNA in molecular biology research.In this paper, their genomic DNA from stem,leaves and endosperm of Pinus armandi Franch were extracted using five methods, which were the classical CTAB method, modified CTAB method, simple CTAB meth-od, SDS micro-extraction method and Zigenhagen method.Then, the extracted DNAs were analyzed by ultraviolet spectrophotometer, agarose gel electrophoresis and standard deviation and standard error of a-rithmetic mean.The results showed:①The modified CTAB method was appropriate for leaves genomic DNA extraction, whose yield was moderate, changes of standard deviation and standard error were lower, and DNA quality was better and stable;②In stem genomic DNA extraction, the yield and the purity of DNA from the Zigenhagen method were highest and its changes of standard deviation and standard error were lowest.Its DNA quality was obviously better and stable;③For endosperm, the yield and the purity of DNA from simple CTAB method was highest, and its changes of standard deviation and standard error were lowest, which indicated that the DNA quality from simple CTAB method was obviously better and stable.Therefore,different methods should be used to extract genomic DNA in the different tissue of one and the same plant.To obtain the best result, the method of DNA extraction need to be explored in prac-tice.