转化医学电子杂志
轉化醫學電子雜誌
전화의학전자잡지
2014年
5期
6-8
,共3页
胸导管结扎%急性出血坏死性胰腺炎%急性肺损伤
胸導管結扎%急性齣血壞死性胰腺炎%急性肺損傷
흉도관결찰%급성출혈배사성이선염%급성폐손상
thoracic duct ligation%thoracic duct drainage%a-cute hemorrhagic necrotizing pancreatitis%acute lung injury
目的：探讨急性出血坏死性胰腺炎引起急性肺损伤（ALI）的发病机制。方法：将成年雄性Wistar大鼠36只随机分为假手术组、急性出血坏死性胰腺炎组和AHNP＋胸导管结扎组三组。采用胰胆管逆行注入牛磺胆酸钠建立急性出血坏死性胰腺炎模型，测定血清肿瘤坏死因子（TNF-α）水平及胰腺、肺脏、小肠组织髓过氧化物酶（MPO）活性，测定大鼠的支气管肺泡灌洗液蛋白含量和TNF-α水平。光镜下观察大鼠肺、小肠、胰腺组织病理损伤情况。结果：AHNP组血清TNF-α水平和支气管肺泡灌洗液蛋白含量均高于假手术组，胰腺、肺脏、小肠MPO活性和病理损伤评分均显著高于假手术组；AH-NP＋胸导管结扎组TNF-α水平和支气管肺泡灌洗液蛋白含量较AHNP组明显降低，肺组织MPO活性和病理损伤评分均显著低于AHNP组，而在小肠和胰腺MPO活性和病理损伤评分较AHNP组升高。结论：腹腔淋巴液是AHNP引起ALI 的可能原因，阻断胸导管淋巴回流可以减轻AHNP引起的急性肺损伤。
目的：探討急性齣血壞死性胰腺炎引起急性肺損傷（ALI）的髮病機製。方法：將成年雄性Wistar大鼠36隻隨機分為假手術組、急性齣血壞死性胰腺炎組和AHNP＋胸導管結扎組三組。採用胰膽管逆行註入牛磺膽痠鈉建立急性齣血壞死性胰腺炎模型，測定血清腫瘤壞死因子（TNF-α）水平及胰腺、肺髒、小腸組織髓過氧化物酶（MPO）活性，測定大鼠的支氣管肺泡灌洗液蛋白含量和TNF-α水平。光鏡下觀察大鼠肺、小腸、胰腺組織病理損傷情況。結果：AHNP組血清TNF-α水平和支氣管肺泡灌洗液蛋白含量均高于假手術組，胰腺、肺髒、小腸MPO活性和病理損傷評分均顯著高于假手術組；AH-NP＋胸導管結扎組TNF-α水平和支氣管肺泡灌洗液蛋白含量較AHNP組明顯降低，肺組織MPO活性和病理損傷評分均顯著低于AHNP組，而在小腸和胰腺MPO活性和病理損傷評分較AHNP組升高。結論：腹腔淋巴液是AHNP引起ALI 的可能原因，阻斷胸導管淋巴迴流可以減輕AHNP引起的急性肺損傷。
목적：탐토급성출혈배사성이선염인기급성폐손상（ALI）적발병궤제。방법：장성년웅성Wistar대서36지수궤분위가수술조、급성출혈배사성이선염조화AHNP＋흉도관결찰조삼조。채용이담관역행주입우광담산납건립급성출혈배사성이선염모형，측정혈청종류배사인자（TNF-α）수평급이선、폐장、소장조직수과양화물매（MPO）활성，측정대서적지기관폐포관세액단백함량화TNF-α수평。광경하관찰대서폐、소장、이선조직병리손상정황。결과：AHNP조혈청TNF-α수평화지기관폐포관세액단백함량균고우가수술조，이선、폐장、소장MPO활성화병리손상평분균현저고우가수술조；AH-NP＋흉도관결찰조TNF-α수평화지기관폐포관세액단백함량교AHNP조명현강저，폐조직MPO활성화병리손상평분균현저저우AHNP조，이재소장화이선MPO활성화병리손상평분교AHNP조승고。결론：복강림파액시AHNP인기ALI 적가능원인，조단흉도관림파회류가이감경AHNP인기적급성폐손상。
AIM:To investigate the pathogenesis of acute hem-orrhagic necrotizing pancreatitis induced acute lung injury(ALI). METHODS:36 male Wistar rats were randomly divided into 3 groups:sham operation group,AHNP group,AHNP +Ligation of thoracic duct group.Rat AHNP model was induced by retro-grade injection of 3.5%sodium deoxycholate into the biliopancre-atic duct.At 6 h after model induction,the bronchoalveolar lav-age fluid (BALF)were collected for determination of tumor nec-rosis factor-alpha (TNF-α),and the tissues of lung,intestine and pancrease were harvested individually for pathohistological e-valuation and the myeloperoxidase (MPO)activity determination in each group.RESULTS:The pathohistological damage and MPO of lung,intestine and pancrease,TNF-αof BALF and ser-um were all increased in AHNP group compared to those in sham operation group.In AHNP +ligation group,the pathohistological damage and MPO of lung and TNF-αof BALF were reduced,but the pathohistological damage and MPO of intestine and pancrease were increased compared with AHNP group.CONCLUSION:Abdominal lymphatic is the pathogenesis of ALI induced by AH-NP,thoracic duct ligation can reduce neutrophil infiltration and TNF -αrelease and then attenuates lung injury in rats with AH-NP.
