CAJ | 학술논문

目的：观察多药耐药基因1（MDR1） C3435T、细胞色素P450（CYP）3A4*18B对维吾尔族、汉族充血性心力衰竭（简称心衰）患者地高辛血药浓度的影响。方法选择维吾尔族、汉族心衰患者各40例，均口服地高辛0．125 mg/（次· d）至少1周。抽取清晨空腹静脉血后，采用PCR-RFLP法检测患者MDR1 C3435T、CYP3A4*18B基因型，日立741-0050全自动生化分析仪检测地高辛血药浓度。结果汉族MDR1 C3435T基因CT、TT型患者地高辛血药浓度均高于 CC 型和维吾尔族同基因型， P 均＜0．05；维吾尔族 MDR1 C3435T 各基因型及两民族CYP3A4*18B各基因型患者地高辛血药浓度比较，P均＞0．05。结论 MDR1 C3435T基因多态性可能会提高汉族心衰患者的地高辛血药浓度，但对维吾尔族患者则无影响；而CYP3A4*18B基因多态性对两民族心衰患者的地高辛血药浓度均没有明显影响。
목적：관찰다약내약기인1（MDR1） C3435T、세포색소P450（CYP）3A4*18B대유오이족、한족충혈성심력쇠갈（간칭심쇠）환자지고신혈약농도적영향。방법선택유오이족、한족심쇠환자각40례，균구복지고신0．125 mg/（차· d）지소1주。추취청신공복정맥혈후，채용PCR-RFLP법검측환자MDR1 C3435T、CYP3A4*18B기인형，일립741-0050전자동생화분석의검측지고신혈약농도。결과한족MDR1 C3435T기인CT、TT형환자지고신혈약농도균고우 CC 형화유오이족동기인형， P 균＜0．05；유오이족 MDR1 C3435T 각기인형급량민족CYP3A4*18B각기인형환자지고신혈약농도비교，P균＞0．05。결론 MDR1 C3435T기인다태성가능회제고한족심쇠환자적지고신혈약농도，단대유오이족환자칙무영향；이CYP3A4*18B기인다태성대량민족심쇠환자적지고신혈약농도균몰유명현영향。
Objective To investigate the effects of MDR1 C3435T and CYP3A4*18B polmorphisms on serum digoxin concentration in Uygurs and Hans nationality patients with heart failure.Methods 40 Uygur nationality patients and 40 Han nationality patients with heart failure were included in this study, the genetyping of MDR1 C3435T and CYP3A4*18B were determined by the polymerase chain reaction with the restriction fragment length polymorphism ( PCR-RFLP) , and to analysis the digoxin concentration by genetic polymorphisms.Results The serum digoxin concentration of MDR1 C3435T genetypes CC group and TT group in Han nationality patients were obviously higher than the same genotype groups in Uygurs nationality patients (all P<0.05).The CYP3A4*18B genetic polymorphism had no significant difference between two groups (P>0.05).Conclusions The serum digoxin concentration is effected by MDR1 C3435T genetic polymor-phism in Han nationality patients with heart failure, but without effect on Uygurs nationality patients with heart failure. CYP3A4*18B genetic polymorphism may have no significant effects on the serum digoxin concentration in two nationalities patients with heart failure.