CAJ | 학술논문

目的：观察缺氧模拟物去铁胺（DFO）对人源肠道Caco-2细胞成纤维细胞生长因子21（FGF21）表达的影响。方法分别用50、100、150、200μmol/L的DFO处理Caco-2细胞12 h，同时用200μmol/L的DFO处理Caco-2细胞4、8、12 h，RT-PCR法检测细胞中的FGF21 mRNA。分别用缺氧诱导因子（ HIF）抑制剂和ROS抑制剂预处理Caco-2细胞，再用DFO继续处理，RT-PCR法检测细胞中的FGF-21 mRNA。结果随DFO浓度增加和作用时间延长，Caco-2细胞FGF21 mRNA相对表达量逐渐降低（P均＜0．05）。 HIF抑制剂预处理后，Caco-2细胞FGF21相对表达量依然降低（P均＜0．05）；ROS抑制剂预处理后，Caco-2细胞FGF21 mRNA相对表达量未出现明显降低（P均＞0．05）。结论 DFO对肠道Caco-2细胞FGF21的表达有抑制作用，该作用与缺氧产生HIF无关，而与ROS有关。
목적：관찰결양모의물거철알（DFO）대인원장도Caco-2세포성섬유세포생장인자21（FGF21）표체적영향。방법분별용50、100、150、200μmol/L적DFO처리Caco-2세포12 h，동시용200μmol/L적DFO처리Caco-2세포4、8、12 h，RT-PCR법검측세포중적FGF21 mRNA。분별용결양유도인자（ HIF）억제제화ROS억제제예처리Caco-2세포，재용DFO계속처리，RT-PCR법검측세포중적FGF-21 mRNA。결과수DFO농도증가화작용시간연장，Caco-2세포FGF21 mRNA상대표체량축점강저（P균＜0．05）。 HIF억제제예처리후，Caco-2세포FGF21상대표체량의연강저（P균＜0．05）；ROS억제제예처리후，Caco-2세포FGF21 mRNA상대표체량미출현명현강저（P균＞0．05）。결론 DFO대장도Caco-2세포FGF21적표체유억제작용，해작용여결양산생HIF무관，이여ROS유관。
Objective To study the effect of hypoxia-mimic deferoxamine ( DFO ) on fibroblast growth factor (FGF21) expression in intestinal Caco-2 cells.Methods Caco-2 cells were either treated with 50, 100, 150, 200μmol/L DFO for 12 h or treated with 200 μmol/L DFO for 4, 8, 12 h.FGF21 mRNA expression were measured by RT-PCR. Caco-2 cells were pretreated with either HIF or ROS inhibitor and then treated with DFO.FGF21 mRNA expression was measured by RT-PCR.Results FGF21 mRNA expression decreased in dose-and time-dependent manners with DFO treat-ment in Caco-2 cells.HIF inhibitor and DFO treatment still decreased FGF21 mRNA expression (all P<0.05).But ROS inhibitor pretreatment reversed DFO-induced FGF21 mRNA down-regulation (all P>0.05).Conclusion DFO-induced FGF21 down-regulation is not associated with HIF stabilization, but involves hypoxia-induced oxidative stress.