CAJ | 학술논문

目的：观察氯沙坦短期用药对高血压前期大鼠血压和心肌的影响。方法24只4周龄雄性自发性高血压大鼠均分3组，A组给生理盐水（NS），B组于4周龄时给20 mg/kg氯沙坦，C组于12周龄时给20 mg/kg氯沙坦；另取8只4周龄雄性京都种Wistar大鼠为D组，给NS作为正常对照。各组均每天1次灌胃给药持续4周，停药后随访观察至24周。各鼠分别于4、8、12、16、20及24周龄时采用尾套法测尾动脉收缩压（SBP）；24周龄时断头取标本，称质量法测右心室质量/体质量比（RVW/BW）全心质量/体质量比（HW/BW）、左心室质量/体质量比（LVW/BW）；苏木素-伊红（HE）染色法观察心肌组织形态学变化，测心肌细胞横断面面积；放射免疫法测血浆与心肌的血管紧张素Ⅱ（AngⅡ）和醛固酮（Aldo）水平。结果 SBP在8周龄时 A、C组明显高于 D组（P＜0．05）；B组明显低于A组（P＜0．05），停药后，B组SBP上升缓慢，直至24周龄时均持续低于A组（P＜0．05），也明显低于C组（P＜0．05）；C组16周龄时SBP明显低于A组（P＜0．05），但仍明显高于D组，且在停药后迅速上升，至24周龄时与 A组比较差异无统计学意义（P＞0．05）。LVW/BW、HW/BW及心肌细胞面积，A组较 D组明显增加（P＜0．05），B、C组较 A组明显减少或减小（P＜0．05），B组较C组明显减少或减小（P＜0．05）。心肌细胞横断面切片光镜下观察，A组较D组心肌细胞肥大、细胞间距增宽和间质纤维增生明显，B组与D组近似，C组介于B组与A组之间。血浆与心肌的 AngⅡ和 Aldo水平，A组较 D组明显增高（P＜0．05），B组较A、C组组明显降低（P＜0．05）。结论氯沙坦短期用于高血压前期大鼠可获取降压和抗心室重构的后效应。
목적：관찰록사탄단기용약대고혈압전기대서혈압화심기적영향。방법24지4주령웅성자발성고혈압대서균분3조，A조급생리염수（NS），B조우4주령시급20 mg/kg록사탄，C조우12주령시급20 mg/kg록사탄；령취8지4주령웅성경도충Wistar대서위D조，급NS작위정상대조。각조균매천1차관위급약지속4주，정약후수방관찰지24주。각서분별우4、8、12、16、20급24주령시채용미투법측미동맥수축압（SBP）；24주령시단두취표본，칭질량법측우심실질량/체질량비（RVW/BW）전심질량/체질량비（HW/BW）、좌심실질량/체질량비（LVW/BW）；소목소-이홍（HE）염색법관찰심기조직형태학변화，측심기세포횡단면면적；방사면역법측혈장여심기적혈관긴장소Ⅱ（AngⅡ）화철고동（Aldo）수평。결과 SBP재8주령시 A、C조명현고우 D조（P＜0．05）；B조명현저우A조（P＜0．05），정약후，B조SBP상승완만，직지24주령시균지속저우A조（P＜0．05），야명현저우C조（P＜0．05）；C조16주령시SBP명현저우A조（P＜0．05），단잉명현고우D조，차재정약후신속상승，지24주령시여 A조비교차이무통계학의의（P＞0．05）。LVW/BW、HW/BW급심기세포면적，A조교 D조명현증가（P＜0．05），B、C조교 A조명현감소혹감소（P＜0．05），B조교C조명현감소혹감소（P＜0．05）。심기세포횡단면절편광경하관찰，A조교D조심기세포비대、세포간거증관화간질섬유증생명현，B조여D조근사，C조개우B조여A조지간。혈장여심기적 AngⅡ화 Aldo수평，A조교 D조명현증고（P＜0．05），B조교A、C조조명현강저（P＜0．05）。결론록사탄단기용우고혈압전기대서가획취강압화항심실중구적후효응。
Objective To observe the influence of losartan on rats′blood pressure and myocardium.Methods To randomly divide 24 4-year-old spontaneously hypertensive male rats into 3 groups.Group A is given normal saline(NS);group B is given losartan 20 mg/kg in 4-week-old;group C is given the same dose in 1 2-week-old;To take another 4-week-old male wistar rats to form group D,feed NS served as normal comparison.Each group was given NS or losartan one time each day through intragastric administration for 4 weeks.Follow up 24 weeks after stopping the medicine.Rats’SBP were measured through tail cuff method respectively when at 4,8,12,16,20 and 24 weeks’ time.After 24 weeks,cut off the head to take the sample,the RVW/BW,HW/BW,LVW/BW were measured through weighing method.To observe the morphologic change of the cardiac muscles and measure the area of myocardium cell cross section through HE stain method.Ra-dioimmunoassay was used to measure the AngⅡand Aldo level of the blood plasma and myocardium.Results At the age of eight-week,the SBP of group A and C are higher than those of group D(P<0.05),group B significantly lower than group A.After stopping the medicine, SBP of group B is slowly increasing and still lower than group A(P<0.05)as well as group C (P<0.05)until the 24th week.At the 16th week,SBP of group C is significantly lower than that of group A(P<0.05)but higher than group D,but quickly increases after stopping the medicine till the 24th week and there is no significant difference between group A and C(P>0.05).About LVW/BW,HW/BW and the ar-ea of myocardium cell cross section,group A increased significantly in comparison with group D (P<0.05),group B and C decreased in comparison with group A(P<0.05),group B decreased significantly in comparison with group C(P<0.05).Through the observation of the area of myocardial cell cross section under the microscopy,group A increased significantly in comparison with group D about myocardial cell fattened and the gap between cells are widened,the fibers of mesenchyma proliferates.group B and D are similar,group C is between group B and A.The level of AngⅡand Aldo in plasma and myocardium of group A increased significantly in comparison with group D (P<0.05),group B decreased significantly in comparison with group A and C(P<0.05).Conclusion To use losartan for a short time in trea-ting rats with prehypertension can get the after-effect of reducing blood pressure and anti-ventricular-remodeling.