CAJ | 학술논문

目的：观察卵巢切除术及激素替代疗法对雌兔膀胱功能的影响。方法选择雌性大白兔21只，随机分为3组。第一组为对照组，假手术，手术后1周起每只动物皮下注射生理盐水，共8周。第二组为卵巢切除组，单纯行双侧卵巢切除术，手术后1周起每只动物皮下注射生理盐水。第三组为雌激素组，行双侧卵巢切除术，手术后1周起每只动物皮下注射17-β雌二醇。8周后每只动物取血，测定血清雌二醇的浓度。动物麻醉后，应用尿流动力检测仪检测膀胱容量、逼尿肌压力、膀胱顺应性。检测后取出膀胱，称膀胱湿质量。取膀胱平滑肌组织，测量记录平滑肌张力的变化。试验后，每条平滑肌标本称质量。结果卵巢切除组的血清雌二醇水平、膀胱容量和膀胱顺应性相对于对照组减少，膀胱湿质量和逼尿肌压力与对照组比较差异无统计学意义。雌激素组的血清雌二醇水平、膀胱湿质量和膀胱容量较对照组显著增加，而膀胱顺应性和逼尿肌压力与对照组比较差异无统计学意义。卵巢切除组的膀胱平滑肌组织对2 Hz电刺激的反应较对照组显著减小。经过8周雌激素治疗的动物膀胱平滑肌组织对2 Hz电刺激的反应较对照组和卵巢切除组均显著增加。膀胱平滑肌组织对8 Hz及32 Hz电刺激的反应与对2 Hz电刺激的反应是一致的。膀胱平滑肌组织对卡巴胆碱、ATP及KCl反应与对电刺激的反应是一致的。结论卵巢切除使雌兔血清雌二醇水平显著降低，膀胱容量和顺应性下降，膀胱平滑肌条对电刺激、卡巴胆碱、ATP、KCl诱导的收缩反应减低。雌激素可逆转这些反应，增加膀胱湿质量。雌激素水平的变化对膀胱功能有显著影响。
목적：관찰란소절제술급격소체대요법대자토방광공능적영향。방법선택자성대백토21지，수궤분위3조。제일조위대조조，가수술，수술후1주기매지동물피하주사생리염수，공8주。제이조위란소절제조，단순행쌍측란소절제술，수술후1주기매지동물피하주사생리염수。제삼조위자격소조，행쌍측란소절제술，수술후1주기매지동물피하주사17-β자이순。8주후매지동물취혈，측정혈청자이순적농도。동물마취후，응용뇨류동력검측의검측방광용량、핍뇨기압력、방광순응성。검측후취출방광，칭방광습질량。취방광평활기조직，측량기록평활기장력적변화。시험후，매조평활기표본칭질량。결과란소절제조적혈청자이순수평、방광용량화방광순응성상대우대조조감소，방광습질량화핍뇨기압력여대조조비교차이무통계학의의。자격소조적혈청자이순수평、방광습질량화방광용량교대조조현저증가，이방광순응성화핍뇨기압력여대조조비교차이무통계학의의。란소절제조적방광평활기조직대2 Hz전자격적반응교대조조현저감소。경과8주자격소치료적동물방광평활기조직대2 Hz전자격적반응교대조조화란소절제조균현저증가。방광평활기조직대8 Hz급32 Hz전자격적반응여대2 Hz전자격적반응시일치적。방광평활기조직대잡파담감、ATP급KCl반응여대전자격적반응시일치적。결론란소절제사자토혈청자이순수평현저강저，방광용량화순응성하강，방광평활기조대전자격、잡파담감、ATP、KCl유도적수축반응감저。자격소가역전저사반응，증가방광습질량。자격소수평적변화대방광공능유현저영향。
Objective It is to explore the effects of ovariectomy and estrogen replacement therapy on the bladder function of female rabbits .Methods Twenty one female white rabbits were randomly divided into 3 groups .Group 1 served as control group, received sham operation and 8 weeks’ treatment with normal saline by hypodermic injection in 1 weeks after operation . Group 2 served as ovariectomy group , received ovariectomy and treatment 8 weeks’ treatment with normal saline by hypoder-mic injection in 1 weeks after operation .Group 3 served as estrogen group , received ovariectomy and treatment with 17 -βestradiol by hypodermic injection in 1 weeks after operation .At the end of the 8 weeks’ treatment, blood samples were ob-tained from each rabbit to determine the content of serum estradiol .Then each rabbit was anesthetized to detect bladder capaci-ty, detrusor pressure and compliance by urodynamic testing machine .After urodynamic test the bladder was removed and weighed .The smooth muscle strips were cut from the bladder body to measure the tension change .At the end of the experi-ment the strips were weighed.RusultsIn ovariectomy group, serum estradiol, bladder capacity and bladder compliance were obviously lower than those in control group (P<0.05), but bladder wet weight and detrusor pressure were similar to those of control group (P>0.05).Estrogen administration resulted in a significant increase in serum estradiol , bladder wet weight and bladder capacity compared with control group , except in bladder compliance and detrusor pressure .As for the contractile re-sponses of bladder strips to field stimulation , the bladders from the ovariectomy group showed decreased responses to 2 Hz compared with those from the control group .After 8 weeks'estrogen treatment, the contractile responses to 2Hz increased sig-nificantly compared with those in the control group and ovariectomy group .The contractile responses to 8Hz and 32Hz of blad-der strips were similar with the contractile responses to 2 Hz.The contractile responses to carbachol ,ATP and KCl of bladder strips were similar with the contractile responses to field stimulation .Conclusion While ovariectomy resulted in a significant decrease in serum estradiol of female rabbit , bladder capacity and compliance showed decline , the contractile responses of bladder strips decreased to all forms of stimulation (field stimulation, carbachol, ATP, KCl).Estrogen administration could reverse these effects and increase bladder wet weight .Bladder function can be significantly affected by modulating the circulat-ing estrogen level .