中国社区医师
中國社區醫師
중국사구의사
Chinese Community Doctors
2014年
30期
126-126,128
,共2页
金标法%酶联免疫法%艾滋病%抗-HIV
金標法%酶聯免疫法%艾滋病%抗-HIV
금표법%매련면역법%애자병%항-HIV
Gold immnnochromatography%Euzymelinked immunosorbent assay%Acquired immune deficiency syndrome%Anti-HIV
目的：比较分析金标法与酶联免疫法在抗-HIV测定中的应用效果。方法：2012年1月-2013年12月收集血标本4250份，分别采用金标法和酶联免疫法对血标本进行抗-HIV检测，比较2种方法用于抗-HIV测定中的准确性。结果：初次试验金标法阳性0.49%，酶联免疫法0.59%；确认试验金标法阳性率0.42%，酶联免疫法0.38%；经免疫印迹法检测最终确诊抗-HIV 阳性率0.35%；金标法和酶联免疫法测定抗-HIV 的假阳性率分别为0.07%(3/4250)和0.02%(1/4250)，2种方法阳性率及假阳性率差异均无统计学意义(P＞0.05)。结论：金标法和酶联免疫法均可用于抗-HIV 测定中，临床中可根据具体情况进行互补应用。
目的：比較分析金標法與酶聯免疫法在抗-HIV測定中的應用效果。方法：2012年1月-2013年12月收集血標本4250份，分彆採用金標法和酶聯免疫法對血標本進行抗-HIV檢測，比較2種方法用于抗-HIV測定中的準確性。結果：初次試驗金標法暘性0.49%，酶聯免疫法0.59%；確認試驗金標法暘性率0.42%，酶聯免疫法0.38%；經免疫印跡法檢測最終確診抗-HIV 暘性率0.35%；金標法和酶聯免疫法測定抗-HIV 的假暘性率分彆為0.07%(3/4250)和0.02%(1/4250)，2種方法暘性率及假暘性率差異均無統計學意義(P＞0.05)。結論：金標法和酶聯免疫法均可用于抗-HIV 測定中，臨床中可根據具體情況進行互補應用。
목적：비교분석금표법여매련면역법재항-HIV측정중적응용효과。방법：2012년1월-2013년12월수집혈표본4250빈，분별채용금표법화매련면역법대혈표본진행항-HIV검측，비교2충방법용우항-HIV측정중적준학성。결과：초차시험금표법양성0.49%，매련면역법0.59%；학인시험금표법양성솔0.42%，매련면역법0.38%；경면역인적법검측최종학진항-HIV 양성솔0.35%；금표법화매련면역법측정항-HIV 적가양성솔분별위0.07%(3/4250)화0.02%(1/4250)，2충방법양성솔급가양성솔차이균무통계학의의(P＞0.05)。결론：금표법화매련면역법균가용우항-HIV 측정중，림상중가근거구체정황진행호보응용。
Objective:To compare and analyze the application effect of the gold immnnochromatography and euzymelinked immunosorbent assay in the determination of anti-HIV.Methods:4 250 cases of blood specimen were selected from January 2012 to December 2013.The determination of anti-HIV in the blood specimen respectively used the gold immnnochromatography and euzymelinked immunosorbent assay.The accuracies of two methods in the determination of anti-HIV were compared.Results:The masculine gender of the first check gold immnnochromatography was 0.49%;the euzymelinked immunosorbent assay was 0.59%. The positive rate of the validation test gold immnnochromatography was 0.42%;the euzymelinked immunosorbent assay was 0.38%. The positive rate of the final diagnosis of anti-HIV by western blot method was 0.35% .The false positive rates of the gold immnnochromatography and euzymelinked immunosorbent assay in the determination of anti-HIV were respectively 0.07%(3/4250) and 0.02% (1/4250);there were no differences in the positive rates and the false positive rate between two groups(P>0.05). Conclusion:The gold immnnochromatography and euzymelinked immunosorbent assay can be used in the determination of anti-HIV. They can be complementary application according to the specific situation of clinical.
