CAJ | 학술논문

探讨含脑血康颗粒的培养基对脑出血患者外周血中提取的血管内皮祖细胞（EPCs）的数量及功能的影响。方法采用密度梯度离心法分离出脑出血患者外周血的EPCs，加入含脑血康颗粒的相应培养基中，并根据培养基中脑血康颗粒浓度0，10，50，100 mg/mL分为对照组、低剂量组、中剂量组和高剂量组，分别检测培养基孵育7 d后EPCs的数量及细胞功能。结果与正常组相比，脑出血组的外周血EPCs数目及黏附、迁移、增殖及体外血管形成能力均明显降低（P均＜0．05）；与对照组相比，脑血康各组外周血的EPCs增殖能力及黏附、迁移及体外血管形成能力均明显提高（P均＜0．05）。结论脑血康颗粒能明显增加脑出血患者外周血EPCs的数量，并且改善其功能，增强EPCs的增殖、分化、黏附和迁移能力，能够更快形成血管样结构。
탐토함뇌혈강과립적배양기대뇌출혈환자외주혈중제취적혈관내피조세포（EPCs）적수량급공능적영향。방법채용밀도제도리심법분리출뇌출혈환자외주혈적EPCs，가입함뇌혈강과립적상응배양기중，병근거배양기중뇌혈강과립농도0，10，50，100 mg/mL분위대조조、저제량조、중제량조화고제량조，분별검측배양기부육7 d후EPCs적수량급세포공능。결과여정상조상비，뇌출혈조적외주혈EPCs수목급점부、천이、증식급체외혈관형성능력균명현강저（P균＜0．05）；여대조조상비，뇌혈강각조외주혈적EPCs증식능력급점부、천이급체외혈관형성능력균명현제고（P균＜0．05）。결론뇌혈강과립능명현증가뇌출혈환자외주혈EPCs적수량，병차개선기공능，증강EPCs적증식、분화、점부화천이능력，능구경쾌형성혈관양결구。
Objective:It is to explore the effects of culture medium containing liquid medicine of Naoxuekang granules on the number and the function of endothelial progenitor cells ( EPCs ) isolated from peripheral blood in patients with cerebral hemorrhage .M ethods EPCs was isolated from peripheral blood of patients with cerebral hemorrhage by the method of density gradient centrifugation .According to the Naoxuekang granules concentration (0,10,50,100 mg/mL) added to culture medi-um, the cells were divided into control group , low dose group , middle dose group and high dose group respectively .Those groups were incubated for 7 days,then the number of EPCs and their impact on cell function was observed .Results In cerebral hemorrhage group compared with normal group ,it is observed that the number of EPCs in peripheral blood and the ability of ad-hesion, migration, proliferation and angiogenesis in vitro decreased obviously (P<0.05).In the Naoxuekang groups compared with control group , the number of EPCs in peripheral blood and the ability of adhesion , migration , proliferation and angiogene-sis in vitro increased significantly ( P<0.05).Conclusion Naoxuekang granules can significantly increase the number of EPCs in patients with cerebral hemorrhage , and improve their capabilities of proliferation , differentiation , adhesion , migration and angiogenesis , thus to formulate angiogenesis more faster .