CAJ | 학술논문

目的：研究云南静脉血栓栓塞（VTE）患者是否携带Leiden V因子（FVL）。方法选取2010年1月至2011年6月经我院确诊的VTE患者98例，用限制性片段长度多态性聚合酶链反应（PCR-RFLP）技术和DNA直接测序的方法，检测患者的凝血V因子基因编码区片段单核苷酸多态性。结果在98例VTE患者中，发现3例FVL杂合子携带者。结论本研究在我国VTE患者中发现FVL杂合子，在亚洲人群中较为罕见，其是否与云南地域或环境因素有关，并影响该地区的VTE发病，需要进一步研究。
목적：연구운남정맥혈전전새（VTE）환자시부휴대Leiden V인자（FVL）。방법선취2010년1월지2011년6월경아원학진적VTE환자98례，용한제성편단장도다태성취합매련반응（PCR-RFLP）기술화DNA직접측서적방법，검측환자적응혈V인자기인편마구편단단핵감산다태성。결과재98례VTE환자중，발현3례FVL잡합자휴대자。결론본연구재아국VTE환자중발현FVL잡합자，재아주인군중교위한견，기시부여운남지역혹배경인소유관，병영향해지구적VTE발병，수요진일보연구。
Objective To explore whether patients with venous thromboembolism(VTE)in Yunnan province carried single nucleotide polymorphisms(SNP)of Factor V(FV).Methods From Jan. 2010 to Jul. 2011, 98 VTE patients diagnosed by venography or vascular ultrasound were selected from the First Affiliated Hospital of Kunming Medical University. Restriction fragment length polymorphism polymerase chain reaction(PCR-RFLP)and DNA sequencing were performed to investigate single nucleotide polymorphism of the coding region of Factor V gene.Results Three cases of Factor V Leiden(FVL, G1691A, R506Q) heterozygote carriers were detected in 98 patients.Conclusion FVL heterozygote carriers, which were rare in Asian, were detected in VTE patients in this study. Therefore, the relationship between FVL and Yunnan regional or environmental factors, and the relationship between FVL and VTE need to be futher studied.