CAJ | 학술논문

目的：建立测定人全血中他克莫司浓度的高效液相-质谱联用方法。方法：采用地西泮为内标，色谱柱为Agilent Poroshell 120 SB-C18柱（4.6 mm ×50 mm，2.7μm），流动相为乙腈-0.01 mol·L-1醋酸铵与0.5%甲酸的混合溶液，梯度洗脱，柱温40℃，流速0.7 mL·min-1，进样量5μL；采用多重反应监测（MRM）进行定量，ESI正离子方式进行检测，用于定量分析的监测离子为m/z 821.6→768.5（他克莫司）和m/z 285.1→193.1（内标地西泮）。结果：他克莫司在1.49~29.78 ng·mL-1的浓度范围内线性关系良好，准确度为95.19%~99.02%，批内与批间（3 d）RSD均小于10.00%。结论：该方法简便、快速、灵敏，适用于他克莫司治疗药物监测工作。
목적：건립측정인전혈중타극막사농도적고효액상-질보련용방법。방법：채용지서반위내표，색보주위Agilent Poroshell 120 SB-C18주（4.6 mm ×50 mm，2.7μm），류동상위을정-0.01 mol·L-1작산안여0.5%갑산적혼합용액，제도세탈，주온40℃，류속0.7 mL·min-1，진양량5μL；채용다중반응감측（MRM）진행정량，ESI정리자방식진행검측，용우정량분석적감측리자위m/z 821.6→768.5（타극막사）화m/z 285.1→193.1（내표지서반）。결과：타극막사재1.49~29.78 ng·mL-1적농도범위내선성관계량호，준학도위95.19%~99.02%，비내여비간（3 d）RSD균소우10.00%。결론：해방법간편、쾌속、령민，괄용우타극막사치료약물감측공작。
Objective:To establish the HPLC-MS/MS method for the determination of tacrolimus in human whole blood. Methods: The diazepam was used as internal standard. Tacrolimus was separated on an Agilent Poroshell 120 SB-C18 column (4.6 mm × 50 mm, 2.7μm) maintained at 40℃with acetonitrile-0.01 mol·L-1 ammonium acetate and 0.5%formic acid as mobile phase by gradient elution. The lfow rate was 0.7 mL·min-1, and the sample size was 5μL. Detection was performed with multiple reaction monitoring (MRM) scan mode measured with positive electrospray ionization (ESI+). The monitoring ions of tacrolimus and diazepam were m/z 821.6→768.5 and m/z 285.1→193.1, respectively. Results:Tacrolimus showed a good linear relationship in the range of 1.49-29.78 ng·mL-1. The accuracies were in the range of 95.19%-99.02%, both the inter-run and intra-run RSD (3 d) were less than 10.00%. Conclusion:The method is simple, rapid and sensitive, which can be used for the therapeutic drug monitoring of tacrolimus.