中国马铃薯
中國馬鈴藷
중국마령서
CHINESE POTATO
2014年
5期
273-276
,共4页
吐温80%次氯酸钠%马铃薯%外植体%灭菌
吐溫80%次氯痠鈉%馬鈴藷%外植體%滅菌
토온80%차록산납%마령서%외식체%멸균
Tween 80%sodium hypochlorite%potato%explant%sterilization
升汞是灭菌效果非常好的一种灭菌剂,但它是有剧毒的重金属盐灭菌剂,对人畜有极强的毒性,且容易污染环境。为了寻找对马铃薯外植体安全有效的灭菌剂,试验通过对马铃薯外植体,用次氯酸钠溶液与添加吐温80的次氯酸钠溶液不同浓度、不同时间处理后,与升汞处理相比较成苗率、感染率和褐化率。试验结果表明,加2%吐温80的11%次氯酸钠溶液处理30 min,对马铃薯外植体的灭菌效果最好,其成苗率高于0.1%升汞处理后的成苗率;加2%吐温80的13%次氯酸钠溶液处理20 min后的灭菌效果也较好,成苗率略低于0.1%升汞处理后的成苗率。
升汞是滅菌效果非常好的一種滅菌劑,但它是有劇毒的重金屬鹽滅菌劑,對人畜有極彊的毒性,且容易汙染環境。為瞭尋找對馬鈴藷外植體安全有效的滅菌劑,試驗通過對馬鈴藷外植體,用次氯痠鈉溶液與添加吐溫80的次氯痠鈉溶液不同濃度、不同時間處理後,與升汞處理相比較成苗率、感染率和褐化率。試驗結果錶明,加2%吐溫80的11%次氯痠鈉溶液處理30 min,對馬鈴藷外植體的滅菌效果最好,其成苗率高于0.1%升汞處理後的成苗率;加2%吐溫80的13%次氯痠鈉溶液處理20 min後的滅菌效果也較好,成苗率略低于0.1%升汞處理後的成苗率。
승홍시멸균효과비상호적일충멸균제,단타시유극독적중금속염멸균제,대인축유겁강적독성,차용역오염배경。위료심조대마령서외식체안전유효적멸균제,시험통과대마령서외식체,용차록산납용액여첨가토온80적차록산납용액불동농도、불동시간처리후,여승홍처리상비교성묘솔、감염솔화갈화솔。시험결과표명,가2%토온80적11%차록산납용액처리30 min,대마령서외식체적멸균효과최호,기성묘솔고우0.1%승홍처리후적성묘솔;가2%토온80적13%차록산납용액처리20 min후적멸균효과야교호,성묘솔략저우0.1%승홍처리후적성묘솔。
Mercuric chloride is one of the good explant sterilants, but it is poisonous to human beings and animals and contaminates environments due to its heavy metal salt. In order to find a safe and an effective potato explant sterilant, this experiment compared mercuric chloride, sodium hypochlorite and sodium hypochlorite added with Tween 80 in different concentrations and different sterilization time for seedling rate, contamination rate and browning rate of potato explants. The results showed that potato explants treated with 11%sodium hypochlorite added with 2%Tween 80 for 30 min gave higher seedling rate than 0.1%mercuric chloride;13%sodium hypochlorite added with 2%Tween 80 treatment for 20 min also gave acceptable results though the seedling rate was slightly lower than that of the control.