CAJ | 학술논문

利用响应面法找出超声波提取神秘果叶多酚的最佳提取工艺,并考察其抗氧化性。在单因素试验的基础上,建立神秘果叶多酚的二次多项数学模型,1.0 g神秘果叶粉末,在液料比30∶1( mL∶g)条件下,所得到最优提取工艺条件为：丙酮体积分数58%、超声波时间72 min、提取温度48℃,实测得率为6.84%,与预测值(6.82%)基本相符。神秘果叶多酚对ABTS、DPPH和·OH自由基的清除作用均呈现量效关系,半数抑制浓度(IC50)值分别为51.81、13.40和28.91 mg/L,表明神秘果叶多酚具有较强的抗氧化能力。
이용향응면법조출초성파제취신비과협다분적최가제취공예,병고찰기항양화성。재단인소시험적기출상,건립신비과협다분적이차다항수학모형,1.0 g신비과협분말,재액료비30∶1( mL∶g)조건하,소득도최우제취공예조건위：병동체적분수58%、초성파시간72 min、제취온도48℃,실측득솔위6.84%,여예측치(6.82%)기본상부。신비과협다분대ABTS、DPPH화·OH자유기적청제작용균정현량효관계,반수억제농도(IC50)치분별위51.81、13.40화28.91 mg/L,표명신비과협다분구유교강적항양화능력。
The purpose of this paper was to determine the optimum technology and conditions to extract polyphenol from the leaves of Synsepalum dulcificum. Based on the results of single factor experiments, by using the response surface method the S. dulcificum leaves polyphenols mathematical model was established. Meanwhile, the antioxidant activity of the extracted polyphenol was evaluated. The best technical conditions to extract polyphenol from S. dulcificum leaf were obtained as follows:the concentration of acetone solution 58%, ultrasonic time 72 min and extraction temperature 48℃, when the S. dulciticum leaf were 1.0 g and liquid-solid ratio was 30∶1(mL∶g). The predicted polyphenol extraction yield was 6. 82%. Under the optimized condition, the experimental yield was 6. 84% which agreed with its predicted yield. The in vitro antioxidant assay results showed that S. dulcificum leaf polyphenol exhibited a dose-dependent scavenging activity against 2,2′-azinobis (3-ethyl-benzothiazoline-6-sulfonic acid ammonium salt(ABTS) radical, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and ·OH radical. Its hemi-inhibiting concentrations (IC50) were 51. 81,13. 40 and 28. 91 mg/L, respectively. Which suggested that S. dulcificum leaf had high antioxidant activity.