CAJ | 학술논문

通过平板涂布、划线分离、菌落观察等方法,从土耳其倍子提取液自然生长的菌落中分离纯化出11株菌,从中初筛出1#、2#、4#这3株降解没食子单宁的菌株,复筛出1株没食子单宁优良降解菌株1#菌,其没食子单宁降解率达65%以上,发酵液中没食子酸质量浓度为0.0523 g/L,单宁酶活为0.920 U/mL；对1#菌株的发酵条件进行了响应面法优化,结果表明：1#菌最佳产酶条件为培养温度31℃,初始培养基pH值5.0,最适培养时间50 h,在该条件下1#菌单宁酶酶活可达1.170 U/mL,与优化前的最大酶活0.920 U/mL相比,提高了27.2%。
통과평판도포、화선분리、균락관찰등방법,종토이기배자제취액자연생장적균락중분리순화출11주균,종중초사출1#、2#、4#저3주강해몰식자단저적균주,복사출1주몰식자단저우량강해균주1#균,기몰식자단저강해솔체65%이상,발효액중몰식자산질량농도위0.0523 g/L,단저매활위0.920 U/mL；대1#균주적발효조건진행료향응면법우화,결과표명：1#균최가산매조건위배양온도31℃,초시배양기pH치5.0,최괄배양시간50 h,재해조건하1#균단저매매활가체1.170 U/mL,여우화전적최대매활0.920 U/mL상비,제고료27.2%。
3 strains of tannic acid degrading were screened out from the 11 strains which were separated from naturally fermented Turkish galls extract by the methods of spread-plate, streaking and colony observing, etc. One superior tannic acid degrading strain was found, named strain 1#. The degradation rate of tannic acid reached beyond 65 %. In the fermentation broth, the concentration of gallic acid was 0. 052 3 g/mL and the tannase activity was 0. 920 U/mL. Fermentation conditions of strain 1# were optimized by using Response Surface Method. The effect of temperature, initial pH, incubation time was investigated. The results showed that the optimum temperature, initial pH values and incubation time of strain 1# was 31 ℃, 5 and 50 h. Under this condition, the tannase activity was increased from 0. 920 to 1. 170 U/mL, and improved by 27. 2%.