林产化学与工业
林產化學與工業
림산화학여공업
CHEMISTRY AND INDUSTRY OF FOREST PRODUCTS
2014年
5期
73-78
,共6页
半纤维素糖液%生物处理%纯化%木糖%菌株驯化
半纖維素糖液%生物處理%純化%木糖%菌株馴化
반섬유소당액%생물처리%순화%목당%균주순화
hemicellulose hydrolysis liquor%biotreatment%purification%xylose%acclimatization
通过采用生物法处理以消除半纤维素糖液中的葡萄糖、半乳糖等杂糖组分,达到纯化半纤维素糖液,提高木糖含量的目的,可望有效提高木糖的收率。采用单因素试验和中心组合实验优化了玉米芯半纤维素糖液在生物处理时的最佳培养基组分(g/L)为：(NH4)2SO45.7、KH2PO42.0、酵母粉2.4。将木糖母液稀释10倍,在此最佳组分下,采用生物处理24 h,半纤维素糖液中的葡萄糖和半乳糖去除率分别达到100%和67%,木糖质量分数从47%提高到60%。通过进一步对菌株进行逐步驯化,使其可处理仅稀释6倍的高浓度糖液。
通過採用生物法處理以消除半纖維素糖液中的葡萄糖、半乳糖等雜糖組分,達到純化半纖維素糖液,提高木糖含量的目的,可望有效提高木糖的收率。採用單因素試驗和中心組閤實驗優化瞭玉米芯半纖維素糖液在生物處理時的最佳培養基組分(g/L)為：(NH4)2SO45.7、KH2PO42.0、酵母粉2.4。將木糖母液稀釋10倍,在此最佳組分下,採用生物處理24 h,半纖維素糖液中的葡萄糖和半乳糖去除率分彆達到100%和67%,木糖質量分數從47%提高到60%。通過進一步對菌株進行逐步馴化,使其可處理僅稀釋6倍的高濃度糖液。
통과채용생물법처리이소제반섬유소당액중적포도당、반유당등잡당조분,체도순화반섬유소당액,제고목당함량적목적,가망유효제고목당적수솔。채용단인소시험화중심조합실험우화료옥미심반섬유소당액재생물처리시적최가배양기조분(g/L)위：(NH4)2SO45.7、KH2PO42.0、효모분2.4。장목당모액희석10배,재차최가조분하,채용생물처리24 h,반섬유소당액중적포도당화반유당거제솔분별체도100%화67%,목당질량분수종47%제고도60%。통과진일보대균주진행축보순화,사기가처리부희석6배적고농도당액。
Biological treatment was used to remove glucose and galactose in this paper to purify the hemicellulose liquid sugars. Single factor and central composite experiments were used to optimize the medium components in the biological treatment. It was seen that the optimal results were obtained when 5. 7 g/L (NH4)2SO4, 2. 0 g/L KH2PO4 and 2. 4 g/L yeast were applied. The removal of glucose and galactose were 100% and 67%, and the purity of xylose increased from 47% to 60% after the optimal biological treatment. Acclimatization of strain used in the biotreatment of the liquor was also conducted to handle the high sugar consistency liquor which was diluted 6 times.
