CAJ | 학술논문

首次利用氧化葡萄糖酸杆菌全细胞催化方法,在液体振荡反应体系中分别制取葡萄糖、木糖、阿拉伯糖、半乳糖和甘露糖这5种植物单糖对应的单糖酸钙溶液。以高效阴离子交换色谱-脉冲安培检测器( HPAEC-PAD )对它们的纯度进行检测,并采用红外光谱( FT-IR )和电喷雾质谱( ESI-MS)进行了物质的结构解析与鉴定。结果显示,制备所得的5种单糖酸钙的离子色谱检测纯度均大于98%；它们的红外光谱在1600 cm-1处均有去质子化COO-基团的特征吸收；在电喷雾质谱中2种戊糖酸和3种己糖酸的特征离子峰[M-H]-分别为165.1和195.1,与理论值相符。
수차이용양화포도당산간균전세포최화방법,재액체진탕반응체계중분별제취포도당、목당、아랍백당、반유당화감로당저5충식물단당대응적단당산개용액。이고효음리자교환색보-맥충안배검측기( HPAEC-PAD )대타문적순도진행검측,병채용홍외광보( FT-IR )화전분무질보( ESI-MS)진행료물질적결구해석여감정。결과현시,제비소득적5충단당산개적리자색보검측순도균대우98%；타문적홍외광보재1600 cm-1처균유거질자화COO-기단적특정흡수；재전분무질보중2충무당산화3충기당산적특정리자봉[M-H]-분별위165.1화195.1,여이론치상부。
Five calcium aldonates were firstly produced from glucose, xylose, arabinose, galactose and mannose respectively by whole cell catalysis using Gluconobacter oxydans in the shake-flask liquid system. Their purities were detected by high performance anion-exchange chromatography coupled with pulsed amperometric detector ( HPAEC-PAD ) , and the molecular structures were identified by infrared spectroscopy (IR) and electrospray ionization mass spectrometry (ESI-MS). The results indicated that a strong absorbance of COO- deprotonated carboxylic group characteristic peak located at 1600 cm-1 was observed in IR. In ESI-MS spectra, the characteristic molecule-mass peaks in pentonic acids and hexonic acids were identified at 165. 1 and 195. 1, respectively. These values were consistent with the theoretical values.