CAJ | 학술논문

目的：给受试者服用不同浓度的人参提取物达玛烷苷元（Dammarane Sapogenin，DS），探究其对有氧运动后肌糖原恢复的影响及作用机制。方法：本研究采用双盲实验设计，选取9名受试者随机分为安慰剂组（placebo group）、服用 DS 240 mg / day 组（DS240）、服用 DS 480 mg / day 组（DS480）进行试验。受试者持续服用 DS 4周后，进行一次性60分钟、强度为75%VO2 max 的蹬功率自行车运动，运动后立刻给予高糖饮食。于运动后即刻及运动后3小时，分别进行肌肉活检，测定 AKT、GS 磷酸化及蛋白质表达；同时，运动后3小时内每隔30分钟取静脉血测定血糖浓度、胰岛素浓度。结果：DS240组较对照组显著提升运动后肌糖原储存速率（P <0.05）；运动后随着时间延长，DS240组的血糖浓度显著低于安慰剂组（P <0.05），但各组胰岛素浓度无显著性差异；运动后 DS240组显著提高运动后 AKT 磷酸化水平、降低肌糖原合成酶磷酸化、提高肌糖原合成酶的蛋白质表达（P <0.05）。结论：长期补充低剂量达玛烷苷元可能提高人体运动后肌糖原储存速率，其可能机制与提升胰岛素作用及血糖吸收有关；研究结果推测达玛烷苷元可以增加胰岛素敏感度，因而可以将达玛烷苷元作为补剂用以运动竞赛或训练后加速肌糖原恢复。
목적：급수시자복용불동농도적인삼제취물체마완감원（Dammarane Sapogenin，DS），탐구기대유양운동후기당원회복적영향급작용궤제。방법：본연구채용쌍맹실험설계，선취9명수시자수궤분위안위제조（placebo group）、복용 DS 240 mg / day 조（DS240）、복용 DS 480 mg / day 조（DS480）진행시험。수시자지속복용 DS 4주후，진행일차성60분종、강도위75%VO2 max 적등공솔자행차운동，운동후립각급여고당음식。우운동후즉각급운동후3소시，분별진행기육활검，측정 AKT、GS 린산화급단백질표체；동시，운동후3소시내매격30분종취정맥혈측정혈당농도、이도소농도。결과：DS240조교대조조현저제승운동후기당원저존속솔（P <0.05）；운동후수착시간연장，DS240조적혈당농도현저저우안위제조（P <0.05），단각조이도소농도무현저성차이；운동후 DS240조현저제고운동후 AKT 린산화수평、강저기당원합성매린산화、제고기당원합성매적단백질표체（P <0.05）。결론：장기보충저제량체마완감원가능제고인체운동후기당원저존속솔，기가능궤제여제승이도소작용급혈당흡수유관；연구결과추측체마완감원가이증가이도소민감도，인이가이장체마완감원작위보제용이운동경새혹훈련후가속기당원회복。
Objective:To investigate the influence of Dammarane Sapogenin make on glycogen’s re-covery by making subjects ingest different doses of DS. Methods:This study adopted double blind counter - balancing study and 9 subjects were divided random into placebo group,DS240(DS240 mg / day),and DS480( DS 480 mg / day). The subjects did an acute 60 - mins cycling exercise (75% VO2 max)after having taken DS for 4 weeks and subjects would be given high carbohydrate meal immediately after exercise. Muscle biopsy,AKT phosphorylation,GS phosphorylation and pro-tein expression should be performed immediately and 3 - h after exercise. At the same time,Blood Glucose and Blood insulin should be tested every 30 mins in 3 hours after exercise. Results:Com-pared with the placebo group,the muscle glycogen storage rates of DS240 was greater. After aerobic exercise,blood sugar concentration of DS240 was significantly lower than those of placebo group, but the difference of the insulin concentration was not significant. AKT phosphorylation of DS240 group was greater than those of placebo group. After the supplement of DS,phospho - glycogen syn-thase was decreased and the protein expression of muscle glycogen synthase was increased signifi-cantly. Conclusions:Taking lowdose of DS for a long time may improve muscle glycogen storage rates and it shows that DS may increase the sensitivity of insulin,which can make DS be supplements and can be used in sports competitions and the recovery of glycogen after exercise.