CAJ | 학술논문

采用生物活性追踪法，从侧柏内生真菌Chaetomium globosum ZH-32发酵液中分离得到1个具有抑菌活性的化合物---WH-01，经紫外光谱、核磁共振及质谱等鉴定为球毛壳菌素A。抑菌活性测定结果表明：WH-01对枯草芽孢杆菌Bacillus subtilis、蜡状芽孢杆菌Bacillus cereus、金黄色葡萄球菌Staphylococcus aureus和大肠埃希氏菌Escherichia coli有强烈的抑制作用，其最低抑制浓度( MIC)分别为3.13、3.13、1.56和3.13μg/mL；对番茄灰霉病菌Botrytis cinerea和油菜菌核病菌Sclerotinia sclerotiorum菌丝生长的抑制中浓度( EC50)分别为5.36和12.19μg/mL。
채용생물활성추종법，종측백내생진균Chaetomium globosum ZH-32발효액중분리득도1개구유억균활성적화합물---WH-01，경자외광보、핵자공진급질보등감정위구모각균소A。억균활성측정결과표명：WH-01대고초아포간균Bacillus subtilis、사상아포간균Bacillus cereus、금황색포도구균Staphylococcus aureus화대장애희씨균Escherichia coli유강렬적억제작용，기최저억제농도( MIC)분별위3.13、3.13、1.56화3.13μg/mL；대번가회매병균Botrytis cinerea화유채균핵병균Sclerotinia sclerotiorum균사생장적억제중농도( EC50)분별위5.36화12.19μg/mL。
The antimicrobial ingredient ( WH-01 ) of Chaetomium globosum ZH-32 , an endophytic fungus from the leaves of Platycladus orientalis, was obtained by bioassay testing of the extraction from the fermentation broth. The compound was identified as chaetoglobosin A by UV, ESI-MS and NMR techniques, as well as by comparing it with the data published previously. Bioassay results showed that WH-01 had strongly inhibitory activities against Bacillus subtilis, Bacillus cereus, Staphylococcus aureuse, and Escherichia coli, and the minimum inhibitory concentration ( MIC ) values of WH-01 were 3. 13, 3. 13, 1. 56 and 3. 13 μg/mL, respectively. Additionally, WH-01 had excellent inhibitory activities against Botrytis cinerea and Sclerotinia sclerotiorum, and the EC50 values were 5. 36 and 12. 19 μg/mL, respectively.