CAJ | 학술논문

建立了气相色谱法测定秋梨膏中环己基氨基磺酸钠含量的分析方法。对国标GB/T 5009.97-2003《食品中环己基氨基磺酸钠的测定》中的气相色谱法进行优化和改进，针对秋梨膏粘稠、含糖量高的特性，对样品稀释后在酸性、低温环境下与亚硝酸钠发生酯化反应，经毛细管色谱柱分离，由氢火焰离子化检测器测定。方法的检出限为2mg/kg，定量限为7mg/kg。在加标水平为2mg/kg 及0.10、0.50和1.00mg/g 时的平均添加回收率为90.0%-97.5%，相对标准偏差3.31%-9.05%。衍生物的峰面积与样品浓度在0.05-1.00g/L 范围内呈良好的线性关系，相关系数R2=0.9996。该方法操作简便快速，灵敏度高，测定结果准确、可靠，适合秋梨膏中环己基氨基磺酸钠含量的测定。
건립료기상색보법측정추리고중배기기안기광산납함량적분석방법。대국표GB/T 5009.97-2003《식품중배기기안기광산납적측정》중적기상색보법진행우화화개진，침대추리고점주、함당량고적특성，대양품희석후재산성、저온배경하여아초산납발생지화반응，경모세관색보주분리，유경화염리자화검측기측정。방법적검출한위2mg/kg，정량한위7mg/kg。재가표수평위2mg/kg 급0.10、0.50화1.00mg/g 시적평균첨가회수솔위90.0%-97.5%，상대표준편차3.31%-9.05%。연생물적봉면적여양품농도재0.05-1.00g/L 범위내정량호적선성관계，상관계수R2=0.9996。해방법조작간편쾌속，령민도고，측정결과준학、가고，괄합추리고중배기기안기광산납함량적측정。
A method for the determination of sodium cyclamate in autumn pear grease by gas chromatography was developed, which gives optimizations and improvements compared with the gas chromatography method described in GB/T 5009.97-2003-Determination of sodium cyclamate in foods. In light of its characteristics of high-viscosity and high sugar content, we dilute the samples of autumn pear grease first, and then derive them with nitrite sodium under acid and low temperature environment. The cyclohexyl nitrite was separated by capillary chromatographic column and analyzed by flame ionizator detector. The limit of detection is 2mg/kg and the limit of quantitation is 7mg/kg. At 2mg/kg and 0.10, 0.50, 1.00mg/g fortification levels, the average recoveries range from 90.0% to 97.5% with the relative standard deviations from 3.31% to 9.05%. The method shows good linearity over the range assayed 0.05 to 1.00g/L (R2=0.9996). The method is suitable for determination of sodium cyclamate in autumn pear grease, owing to its advantages of rapidity, sensitivity and accuracy.