CAJ | 학술논문

以杂交鹅掌楸体细胞胚为材料，研究体胚不同发育阶段、大量元素和维生素C、基因型、激素组合以及移栽基质对体胚苗萌发和移栽存活的影响。结果表明：鱼雷型胚萌发效果最好；3/4 MS培养基较适合体胚苗的萌发；Vc有利用保持体胚苗维持正常形态。不同基因型体胚苗萌发差异较大，基因型1×5002萌发效果最好，成苗率为84.26%；而1×4088仅有13.24%。 KT 0.1 mg/L+IBA 0.1 mg/L有利于促进体细胞胚的萌发。不同基因型体胚苗移栽存活率有差异，黄心土较混合泥炭土更适合体胚苗移栽。
이잡교아장추체세포배위재료，연구체배불동발육계단、대량원소화유생소C、기인형、격소조합이급이재기질대체배묘맹발화이재존활적영향。결과표명：어뢰형배맹발효과최호；3/4 MS배양기교괄합체배묘적맹발；Vc유이용보지체배묘유지정상형태。불동기인형체배묘맹발차이교대，기인형1×5002맹발효과최호，성묘솔위84.26%；이1×4088부유13.24%。 KT 0.1 mg/L+IBA 0.1 mg/L유리우촉진체세포배적맹발。불동기인형체배묘이재존활솔유차이，황심토교혼합니탄토경괄합체배묘이재。
The influences of development period of somatic embryos, major element and vitamin C, genotypes, different combinations with plant growth regulators and transplant medium on germination and transplanting survival for somatic embryos of Liriodendrom hybrids were studied. The results showed that the optimal developement phase of germination was torpedo-shaped embryos, and the base cultural medium of 3/4 MS and with the vitamin C used of keeping normal form from somatic embryos. The somatic embryo germination among different genotypes at the same cultural condition was different significantly, for example, the genotype 1×5002 was the highest, up to 82.46%, the genotype 1×4088 just was to 13.24%. The medium KT 0.1 mg/L+IBA 0.1mg/L can help to promote the germination of somatic embryogenesis. There were differences of seedling transplanting survival rate between the yellow subsoil and mixed peat soil, and the former was more suitable for transplanting than the later.