传染病信息
傳染病信息
전염병신식
INFECTIOUS DISEASE INFORMATION
2014年
5期
302-305
,共4页
秦建成%徐军%戴亮%赵静%王卫东%李想%张岚%毛远丽
秦建成%徐軍%戴亮%趙靜%王衛東%李想%張嵐%毛遠麗
진건성%서군%대량%조정%왕위동%리상%장람%모원려
干扰素γ释放试验%结核分枝杆菌%预测%免疫印迹法%指示剂和试剂
榦擾素γ釋放試驗%結覈分枝桿菌%預測%免疫印跡法%指示劑和試劑
간우소γ석방시험%결핵분지간균%예측%면역인적법%지시제화시제
interferon-gamma release tests%Mycobacterium tuberculosis%forecasting%immunoblotting%indicators and reagents
目的：评价采用A.TB试剂[酶联免疫吸附试验（enzyme-linked immunosorbent assay, ELISA）]进行结核杆菌特异性γ-干扰素释放试验（interferon-gamma release assays, IGRAs）对结核病的临床诊断性能及应用价值。方法108例研究对象中，临床明确诊断结核病60例（结核组），非结核病对照者48例（非结核对照组）。同时采用A.TB试剂（ELISA法）和T-SPOT. TB试剂[酶联免疫斑点试验（enzyme-linked immunosorbent spot, ELISPOT）]进行IGRAs。结果 A.TB试剂（ELISA法）检测的灵敏度为86.67%，特异度为85.42%，受试者工作特征曲线（receiver-operating characteristic curve, ROC曲线）下面积为0.822，95%可信区间（confidence interval, CI）为0.733～0.912。T-SPOT.TB试剂（ELSPOT法）检测灵敏度为91.67%，特异度为87.50%， ROC曲线下面积为0.884，95%CI为0.806～0.962。ROC曲线下面积差异无统计学意义（χ2=0.081，P=0.776）。2种检测方法一致率为87.04%（94/108），Kappa值为0.738。结论 A.TB试剂和T-SPOT.TB试剂检测的整体诊断性能相似，且A.TB试剂检测在操作和检测结果判读方面明显优于T-SPOT.TB试剂。
目的：評價採用A.TB試劑[酶聯免疫吸附試驗（enzyme-linked immunosorbent assay, ELISA）]進行結覈桿菌特異性γ-榦擾素釋放試驗（interferon-gamma release assays, IGRAs）對結覈病的臨床診斷性能及應用價值。方法108例研究對象中，臨床明確診斷結覈病60例（結覈組），非結覈病對照者48例（非結覈對照組）。同時採用A.TB試劑（ELISA法）和T-SPOT. TB試劑[酶聯免疫斑點試驗（enzyme-linked immunosorbent spot, ELISPOT）]進行IGRAs。結果 A.TB試劑（ELISA法）檢測的靈敏度為86.67%，特異度為85.42%，受試者工作特徵麯線（receiver-operating characteristic curve, ROC麯線）下麵積為0.822，95%可信區間（confidence interval, CI）為0.733～0.912。T-SPOT.TB試劑（ELSPOT法）檢測靈敏度為91.67%，特異度為87.50%， ROC麯線下麵積為0.884，95%CI為0.806～0.962。ROC麯線下麵積差異無統計學意義（χ2=0.081，P=0.776）。2種檢測方法一緻率為87.04%（94/108），Kappa值為0.738。結論 A.TB試劑和T-SPOT.TB試劑檢測的整體診斷性能相似，且A.TB試劑檢測在操作和檢測結果判讀方麵明顯優于T-SPOT.TB試劑。
목적：평개채용A.TB시제[매련면역흡부시험（enzyme-linked immunosorbent assay, ELISA）]진행결핵간균특이성γ-간우소석방시험（interferon-gamma release assays, IGRAs）대결핵병적림상진단성능급응용개치。방법108례연구대상중，림상명학진단결핵병60례（결핵조），비결핵병대조자48례（비결핵대조조）。동시채용A.TB시제（ELISA법）화T-SPOT. TB시제[매련면역반점시험（enzyme-linked immunosorbent spot, ELISPOT）]진행IGRAs。결과 A.TB시제（ELISA법）검측적령민도위86.67%，특이도위85.42%，수시자공작특정곡선（receiver-operating characteristic curve, ROC곡선）하면적위0.822，95%가신구간（confidence interval, CI）위0.733～0.912。T-SPOT.TB시제（ELSPOT법）검측령민도위91.67%，특이도위87.50%， ROC곡선하면적위0.884，95%CI위0.806～0.962。ROC곡선하면적차이무통계학의의（χ2=0.081，P=0.776）。2충검측방법일치솔위87.04%（94/108），Kappa치위0.738。결론 A.TB시제화T-SPOT.TB시제검측적정체진단성능상사，차A.TB시제검측재조작화검측결과판독방면명현우우T-SPOT.TB시제。
Objective To evaluate the diagnostic performance and application value of enzyme-linked immunosorbent assay (ELISA)-based interferon-gamma release assays (IGRAs) with A.TB kit for patients with tuberculosis. Methods Of 108 patients en-rolled in the study, 60 were definitely diagnosed with tuberculosis (tuberculosis group), and 48 were controls (non-tuberculosis group). ELISA-based IGRAs with A.TB kit and enzyme-linked immunosorbent spot (ELISPOT)-based IGRAs with T-SPOT.TB kit were car-ried out simultaneously. Results The sensitivity and specificity of ELISA-based IGRAs with A.TB kit were 86.67% and 85.42%, and the area under a receiver-operating characteristic (ROC) curve was 0.822 [95% confidence interval (CI), 0.733-0.912]. The sensitivity and specificity of ELISPOT-based IGRAs with T-SPOT.TB kit were 91.67% and 87.50%, and the area under ROC curve was 0.884 (95%CI, 0.806-0.962). The difference in the area under ROC curve was not significant between the two assays (χ2=0.081, P=0.776). The consistency of the two assays was 87.04%(94/108) with the Kappa value of 0.738. Conclusions ELISA-based IGRAs with A.TB kit and ELISPOT-based IGRAs with T-SPOT.TB kit have a similar overall diagnostic performance, but ELISA-based IGRAs with A.TB kit is superior to ELISPOT-based IGRAs with T-SPOT.TB kit in the operation and interpretation of the test results.
