CAJ | 학술논문

目的：建立一种适合临床常规检测白细胞介素28B（interleukin-28B, IL-28B）rs12979860基因多态性的快速实验方法。方法随机收集我院223例丙型肝炎住院患者外周血标本，设计针对IL-28B rs12979860基因多态性位点的特异性引物和荧光探针，直接通过双色荧光PCR法检测IL-28B rs12979860基因多态性，以基因测序法进行验证，同时初步探讨IL-28B rs12979860不同基因型在本研究人群中的分布频率。结果双色荧光PCR法能很好地鉴别IL-28B rs129798603种基因型，与测序法结果一致率为100%。IL-28B rs12979860基因多态性中T/T、C/T和C/C基因型在本研究人群中的分布频率分别为1.79%（4/223）、23.32%（52/223）和74.89%（167/223）。结论根据IL-28B rs12979860单核苷酸多态性基因型特异性探针和引物所建立的双色荧光PCR法能快速有效地进行rs12979860基因多态性分型，方法快速、可靠，为丙型肝炎患者抗病毒个体化治疗效果的预测提供了一种有效的检测方法。
목적：건립일충괄합림상상규검측백세포개소28B（interleukin-28B, IL-28B）rs12979860기인다태성적쾌속실험방법。방법수궤수집아원223례병형간염주원환자외주혈표본，설계침대IL-28B rs12979860기인다태성위점적특이성인물화형광탐침，직접통과쌍색형광PCR법검측IL-28B rs12979860기인다태성，이기인측서법진행험증，동시초보탐토IL-28B rs12979860불동기인형재본연구인군중적분포빈솔。결과쌍색형광PCR법능흔호지감별IL-28B rs129798603충기인형，여측서법결과일치솔위100%。IL-28B rs12979860기인다태성중T/T、C/T화C/C기인형재본연구인군중적분포빈솔분별위1.79%（4/223）、23.32%（52/223）화74.89%（167/223）。결론근거IL-28B rs12979860단핵감산다태성기인형특이성탐침화인물소건립적쌍색형광PCR법능쾌속유효지진행rs12979860기인다태성분형，방법쾌속、가고，위병형간염환자항병독개체화치료효과적예측제공료일충유효적검측방법。
Objective To establish a rapid method suitable for routine clinical detection of interleukin (IL)-28B rs12979860 gene polymorphisms. Methods A total of 223 peripheral blood samples from hospitalized patients with hepatitis C in our hospital were randomly collected. The specific primers and fluorescent probe sequences in the sites of IL-28B rs12979860 gene polymor-phisms were designed, and IL-28B rs12979860 gene polymorphisms were detected by two-color fluorescent PCR directly, and vali-dated by gene sequencing method. The frequencies of different HCV IL-28B rs12979860 genotypes in this population were dis-cussed as well. Results The two-color fluorescent PCR technology could identify 3 genotypes of IL-28B rs12979860 single nu-cleotide polymorphisms, achieving 100%consistence with sequencing method. The frequencies of T/T, C/T and C/C genotypes in IL-28B rs12979860 gene polymorphisms were 1.79% (4/223), 23.32% (52/223) and 74.89% (167/223), respectively. Conclusions The two-color fluorescent PCR technology based on the specific probe and primer of IL-28B rs12979860 single nucleotide polymor-phisms is effective, rapid and reliable in identifying genotypes of rs12979860 gene polymorphisms. It is an effective detection method for the prediction of antiviral individualized treatment in patients with hepatitis C.