川北医学院学报
川北醫學院學報
천북의학원학보
JOURNAL OF NORTH SICHUAN MEDICAL COLLEGE
2014年
5期
446-449
,共4页
环氧合酶2抑制剂%卵巢肿瘤%细胞系%生长%血管内皮细胞生长因子
環氧閤酶2抑製劑%卵巢腫瘤%細胞繫%生長%血管內皮細胞生長因子
배양합매2억제제%란소종류%세포계%생장%혈관내피세포생장인자
Cyclooxygenase-2 inhibitor%Ovarian carcinoma%Cell line%Growth%Vascular endothelial growth factor
目的：探讨选择性COX-2抑制剂尼美舒利( Nimesulide)对COX-2表达不同的人卵巢癌细胞株移植瘤生长的影响。方法：培养人卵巢癌细胞株SKOV-3(COX-2表达阴性)和OVCAR-3(COX-2表达阳性),制作裸鼠皮下移植瘤模型。应用尼美舒利干预,观察各组肿瘤的生长情况,绘制生长曲线,计算抑瘤率。测定移植瘤组织内血管内皮细胞生长因子( VEGF)含量。结果： SKOV-3组治疗组的肿瘤体积与对照组比较没有统计学差异(1.0264±0.34)cm3vs(1.6682±0.94)cm3(P=0．19>0.05)；两组肿瘤的VEGF含量比较为没有统计学差异(1481.1±235.61)pg/mL vs(1648±179.02)pg/mL(P=0．09>0．05)；两组的肿瘤生长曲线没有显著分离。 OVCAR-3组治疗组的肿瘤体积(0.1396±0.061) cm3小于对照组的肿瘤体积(0．2634±0.056)cm3(P=0.01),抑瘤率为56.3%；治疗组的肿瘤生长曲线较对照组低平。治疗组肿瘤的 VEGF含量为(1070.4±578.29)pg/mL,低于对照组肿瘤的VEGF含量(1641.3±541.84)pg/mL(P=0.04)。结论：选择性COX-2抑制剂对COX-2表达阳性的人卵巢癌组织有抑制生长的作用,对COX-2表达阴性的人卵巢癌组织的生长没有表现出抑制作用,这可能与COX-2抑制剂对COX-2表达不同的肿瘤组织内VEGF含量有不同的影响相关。
目的：探討選擇性COX-2抑製劑尼美舒利( Nimesulide)對COX-2錶達不同的人卵巢癌細胞株移植瘤生長的影響。方法：培養人卵巢癌細胞株SKOV-3(COX-2錶達陰性)和OVCAR-3(COX-2錶達暘性),製作裸鼠皮下移植瘤模型。應用尼美舒利榦預,觀察各組腫瘤的生長情況,繪製生長麯線,計算抑瘤率。測定移植瘤組織內血管內皮細胞生長因子( VEGF)含量。結果： SKOV-3組治療組的腫瘤體積與對照組比較沒有統計學差異(1.0264±0.34)cm3vs(1.6682±0.94)cm3(P=0．19>0.05)；兩組腫瘤的VEGF含量比較為沒有統計學差異(1481.1±235.61)pg/mL vs(1648±179.02)pg/mL(P=0．09>0．05)；兩組的腫瘤生長麯線沒有顯著分離。 OVCAR-3組治療組的腫瘤體積(0.1396±0.061) cm3小于對照組的腫瘤體積(0．2634±0.056)cm3(P=0.01),抑瘤率為56.3%；治療組的腫瘤生長麯線較對照組低平。治療組腫瘤的 VEGF含量為(1070.4±578.29)pg/mL,低于對照組腫瘤的VEGF含量(1641.3±541.84)pg/mL(P=0.04)。結論：選擇性COX-2抑製劑對COX-2錶達暘性的人卵巢癌組織有抑製生長的作用,對COX-2錶達陰性的人卵巢癌組織的生長沒有錶現齣抑製作用,這可能與COX-2抑製劑對COX-2錶達不同的腫瘤組織內VEGF含量有不同的影響相關。
목적：탐토선택성COX-2억제제니미서리( Nimesulide)대COX-2표체불동적인란소암세포주이식류생장적영향。방법：배양인란소암세포주SKOV-3(COX-2표체음성)화OVCAR-3(COX-2표체양성),제작라서피하이식류모형。응용니미서리간예,관찰각조종류적생장정황,회제생장곡선,계산억류솔。측정이식류조직내혈관내피세포생장인자( VEGF)함량。결과： SKOV-3조치료조적종류체적여대조조비교몰유통계학차이(1.0264±0.34)cm3vs(1.6682±0.94)cm3(P=0．19>0.05)；량조종류적VEGF함량비교위몰유통계학차이(1481.1±235.61)pg/mL vs(1648±179.02)pg/mL(P=0．09>0．05)；량조적종류생장곡선몰유현저분리。 OVCAR-3조치료조적종류체적(0.1396±0.061) cm3소우대조조적종류체적(0．2634±0.056)cm3(P=0.01),억류솔위56.3%；치료조적종류생장곡선교대조조저평。치료조종류적 VEGF함량위(1070.4±578.29)pg/mL,저우대조조종류적VEGF함량(1641.3±541.84)pg/mL(P=0.04)。결론：선택성COX-2억제제대COX-2표체양성적인란소암조직유억제생장적작용,대COX-2표체음성적인란소암조직적생장몰유표현출억제작용,저가능여COX-2억제제대COX-2표체불동적종류조직내VEGF함량유불동적영향상관。
Objective:To evaluate the different effects of a selective COX-2 inhibitor nimesulide on the growth of cell line of ovarian carcinoma xenografted tumor. Methods:The cell lines SKOV-3 and OVCAR-3 were cultured in RPMI 1640,and the mice model was built by them. To measure the volume of tumors during the study,and draw the growth curve. The volume of tumors of nimesulide-group was compared with that of the control-group,and the vascular endothelial growth factor( VEGF) levels in these tumors were measured. Results:SKOV-3 Group:Average tumor volume of control mice was(1. 668 2 ± 0. 94)cm3,and that of treated mice was(1. 026 4 ± 0.34)cm3.Therewasnodifferencebetweenthem(P=0.19>0.05).TheexpressionofVEGFwas(1481.1±235.61)pg/mLintrea-ted mice,while(1 648 ± 179. 02)pg/mL in control mice(P=0. 09>0. 05). The growth curves from two groups didn’t show the separate tendency.OVCAR-3Group:Averagetumorvolumeofcontrolmice(0.2634±0.056cm3)wasbiggerthanthat(0.1396±0.061cm3) of treated mice(P=0. 01<0. 05). The inhibitory rate of tumor growth is 56. 3%. Compared to control mice,the growth curve of treated mice was much lower and flatter. The expression of VEGF was significantly down regulated in the nimesulide-treated group:(1 070. 4 ± 578. 29 pg/mL)vs(1 641. 3 ± 541. 84 pg/mL). Conclusion:The study implies that selective COX-2 inhibitors can inhibit COX-2-pos-tive tumor growth. There is no evidence to support that COX-2 inhibitors can inhibit COX-2-negative tumor. These findings might be cor-related with the treat-induced diversities of VEGF expression in different COX-2 profiles which lead to angiogenesis.
