中国骨质疏松杂志
中國骨質疏鬆雜誌
중국골질소송잡지
CHINESE JOURNAL OF OSTEOPOROSIS
2014年
9期
1075-1078,1096
,共5页
匡威%谭家莉%段建民%汪维健%李潇%王桥%李洪涛%刘琴瑶
劻威%譚傢莉%段建民%汪維健%李瀟%王橋%李洪濤%劉琴瑤
광위%담가리%단건민%왕유건%리소%왕교%리홍도%류금요
骨髓间充质干细胞%miR203%增殖
骨髓間充質榦細胞%miR203%增殖
골수간충질간세포%miR203%증식
Bone marrow mesenchymal stem cells%MiR203%Proliferation
目的:探索不同年龄骨髓间充质干细胞中miR203的表达变化,及其对骨髓间充质干细胞自我更新的作用机制。方法分别分离培养4周龄和18~24月龄的Balb/c小鼠BMSCs,对比不同年龄小鼠BMSCs增殖潜能的差异,并检测不同年龄小鼠BMSCs中miR-203的表达变化差异,从而探讨miR-203在骨髓间充质干细胞增殖调节中的作用机制。结果根据干细胞贴壁特性获得了稳定的骨髓间充质干细胞,其在分化诱导条件下可获得经茜素红染色呈红色结节及油红O 染色显示有脂质沉淀,且成骨诱导后Ⅰ型胶原蛋白显著表达。在增殖条件下,与年轻BMSCs相比,老年BMSCs增殖(传代)能力明显下降。年轻小鼠(4周龄)BMSCs中miR-203远低于老年小鼠(18~24月龄)BMSCs中miR-203表达(P<0.05)。结论年轻骨髓间充质干细胞增殖能力优于老年骨髓间充质干细胞,可能与miR-203表达较低有关。
目的:探索不同年齡骨髓間充質榦細胞中miR203的錶達變化,及其對骨髓間充質榦細胞自我更新的作用機製。方法分彆分離培養4週齡和18~24月齡的Balb/c小鼠BMSCs,對比不同年齡小鼠BMSCs增殖潛能的差異,併檢測不同年齡小鼠BMSCs中miR-203的錶達變化差異,從而探討miR-203在骨髓間充質榦細胞增殖調節中的作用機製。結果根據榦細胞貼壁特性穫得瞭穩定的骨髓間充質榦細胞,其在分化誘導條件下可穫得經茜素紅染色呈紅色結節及油紅O 染色顯示有脂質沉澱,且成骨誘導後Ⅰ型膠原蛋白顯著錶達。在增殖條件下,與年輕BMSCs相比,老年BMSCs增殖(傳代)能力明顯下降。年輕小鼠(4週齡)BMSCs中miR-203遠低于老年小鼠(18~24月齡)BMSCs中miR-203錶達(P<0.05)。結論年輕骨髓間充質榦細胞增殖能力優于老年骨髓間充質榦細胞,可能與miR-203錶達較低有關。
목적:탐색불동년령골수간충질간세포중miR203적표체변화,급기대골수간충질간세포자아경신적작용궤제。방법분별분리배양4주령화18~24월령적Balb/c소서BMSCs,대비불동년령소서BMSCs증식잠능적차이,병검측불동년령소서BMSCs중miR-203적표체변화차이,종이탐토miR-203재골수간충질간세포증식조절중적작용궤제。결과근거간세포첩벽특성획득료은정적골수간충질간세포,기재분화유도조건하가획득경천소홍염색정홍색결절급유홍O 염색현시유지질침정,차성골유도후Ⅰ형효원단백현저표체。재증식조건하,여년경BMSCs상비,노년BMSCs증식(전대)능력명현하강。년경소서(4주령)BMSCs중miR-203원저우노년소서(18~24월령)BMSCs중miR-203표체(P<0.05)。결론년경골수간충질간세포증식능력우우노년골수간충질간세포,가능여miR-203표체교저유관。
Objective To investigate the differential expression of miR203 in bone marrow mesenchymal stem cells ( BMSCs) with different age, and to explore the mechanism of miR203 during the self-renew of BMSCs.Methods BMSCs from 4-week and 18-24-month Balb/c mice were isolated and cultured.The proliferation potential of BMSCs in mice with different age was compared.And the different expression level of miR-203 in BMSCs with different age was also detected, in order to identify the mechanism of miR-203 in regulating the proliferation of BMSCs.Results BMSCs were acquired and stably cultured in vitro using adherent separation method.After the induction of BMSCs, the result of Alizarin red staining showed salmon tubercles in BMSCs, while the result of oil red O staining showed lipid sediment in BMSCs.After the osteogenic induction, the significant expression of type I collagen was observed.The capability of proliferation of aged BMSCs was distinctively decreased compared with that of young BMSCs during proliferation.The expression of miR-203 in BMSCs from young mice (4 weeks) was significantly lower than that in BMSCs from the aged mice ( 18 -24 months, P<0.05 ) .Conclusion The capability of proliferation in BMSCs from young mice is superior to that in BMSCs from the aged mice, which may be related to the low expression level of miR-203.