CAJ | 학술논문

1960年，美国骨整形外科医生Harold Frost提出一种定量研究骨微结构和骨量的技术---骨组织形态计量学（BHMT），其采用不脱钙硬包埋法和活体荧光标记法进行骨组织标本制作，更好地保留了骨组织微细结构。50多年来的实践证实BHMT是目前评价骨转换与骨矿化最常用和有效的实验手段，至今仍是骨代谢和矿化评价的金标准。目前国际上有两套常用的标准骨组织形态计量学方法：一是Harold Frost与美国犹他大学医学院放射生物系Webster S．S．Jee教授及Sun Valley Hard Tissue Workshops一起发展并提出的BHMT标准命名法，下文简称Dr．Frost and Jee 教授团队命名法。二是在美国医生A． Michael Parfitt于1980年提出的一套强调骨组织微结构研究重要性的体视学测量方法基础上，由美国骨矿研究协会（ ASBMR）综合提出的标准命名法（1987年第一版和2012年第二版）。上述两种系统命名法均使用广泛，每套系统的指标都很多且各有侧重，使用时可按具体实验需要选择最为适用的系统。本文将讨论2种方法的异同点及其在BHMT研究中的应用。
1960년，미국골정형외과의생Harold Frost제출일충정량연구골미결구화골량적기술---골조직형태계량학（BHMT），기채용불탈개경포매법화활체형광표기법진행골조직표본제작，경호지보류료골조직미세결구。50다년래적실천증실BHMT시목전평개골전환여골광화최상용화유효적실험수단，지금잉시골대사화광화평개적금표준。목전국제상유량투상용적표준골조직형태계량학방법：일시Harold Frost여미국유타대학의학원방사생물계Webster S．S．Jee교수급Sun Valley Hard Tissue Workshops일기발전병제출적BHMT표준명명법，하문간칭Dr．Frost and Jee 교수단대명명법。이시재미국의생A． Michael Parfitt우1980년제출적일투강조골조직미결구연구중요성적체시학측량방법기출상，유미국골광연구협회（ ASBMR）종합제출적표준명명법（1987년제일판화2012년제이판）。상술량충계통명명법균사용엄범，매투계통적지표도흔다차각유측중，사용시가안구체실험수요선택최위괄용적계통。본문장토론2충방법적이동점급기재BHMT연구중적응용。
As a quantitative research technique of bone microstructure and bone mass, bone histomorphometry ( BHMT) has been proposed by Harold Frost, an American orthopedic surgeon, in 1960.For better preservation of bone microstructure, undecalcified embedding and fluorescent labeling in vivo are used to make bone tissue samples for BHMT.More than fifty-year application has proved that BHMT is not only the most common and effective method used for the evaluation of bone turnover and bone mineralization, but also the golden standard for the evaluation of bone metabolism and mineralization.There are two common bone histomorphometry methods.One is proposed by Harold Frost and Webster S.S.Jee, a professor from the Department of Radiation Biology, the Medical College of Utah University, and Sun Valley Hard Tissue Workshops ( abbreviated as BHMT nomenclature system).Another method is developed by the American Society for Bone and Mineral Research (ASBMR), based on a set of stereological techniques for bone microarchitecture measurement, which was created by Dr.Parfitt in 1980.Both nomenclature systems have their own parameters and focus, and are widely used.It needs to choose the most appropriate system according to the particular circumstances.In this paper, we discuss the similarities and differences between the two bone histomorphometry methods, and introduce their main application.