CAJ | 학술논문

目的：研究一种具有生长激素释放活性的脑肠肽（Ghrelin）对大鼠心室肌细胞L型钙电流的影响。方法：采用酶解消化法获得大鼠单个心室肌细胞，通过全细胞膜片钳技术研究不同浓度（10 nmol/L、100 nmol/L及1μmol/L） Ghrelin对L型钙电流的影响。结果：10 nmol/L、100 nmol/L及1μmol/L Ghrelin依次抑制大鼠心室肌细胞L型钙电流峰电位，抑制率分别为：8.95%±2.13%、31.18%±4.78%及64.63%±8.57%，（P<0.05）；I-V曲线上移，通道半数失活电压从（-1.34±1.9） mV分别降至（-8.04±1.32）mV、（9.76±1.17）mV及（-11.81±0.73）mV（P<0.05），并延长失活后恢复时间由给药前τ值63.23±9.32，分别增至98.95±10.74、109.56±13.42和127.39±16.13，差异有统计学意义（P<0.05）。结论：Ghrelin通过加快L型钙电流的失活及延长失活后恢复时间，具有浓度依赖性地抑制L型钙电流。
목적：연구일충구유생장격소석방활성적뇌장태（Ghrelin）대대서심실기세포L형개전류적영향。방법：채용매해소화법획득대서단개심실기세포，통과전세포막편겸기술연구불동농도（10 nmol/L、100 nmol/L급1μmol/L） Ghrelin대L형개전류적영향。결과：10 nmol/L、100 nmol/L급1μmol/L Ghrelin의차억제대서심실기세포L형개전류봉전위，억제솔분별위：8.95%±2.13%、31.18%±4.78%급64.63%±8.57%，（P<0.05）；I-V곡선상이，통도반수실활전압종（-1.34±1.9） mV분별강지（-8.04±1.32）mV、（9.76±1.17）mV급（-11.81±0.73）mV（P<0.05），병연장실활후회복시간유급약전τ치63.23±9.32，분별증지98.95±10.74、109.56±13.42화127.39±16.13，차이유통계학의의（P<0.05）。결론：Ghrelin통과가쾌L형개전류적실활급연장실활후회복시간，구유농도의뢰성지억제L형개전류。
Objective: To study the effect of ghrelin on L-type calcium channel current (ICa-L) of ventricular myocytes in experimental rats. Methods: The single ventricular myocyte in experimental rats were obtained by enzymolysis method, and the whole-cell patch-clamp technique was used to investigate the effect of ghrelin on ICa-L of ventricular myocytes at different doses of 10 nmol/L, 100 nmol/L and 1μmol/L respectively. Results: Ghrelin at 10 nmol/L, 100 nmol/L and 1μmol/L may inhibit ICa-L at (8.95 ± 2.13) %, (31.18 ± 4.78) % and (64.63 ± 8.57)% respectively,P<0.05, and the current-voltage curve was shifted upwards. The channel half inactivation curve decreased from (-1.34 ± 1.9) mV to (-8.04 ± 1.32 ) mV, (9.76 ± 1.17) mV and (-11.81 ± 0.73) mV respectively,P<0.05, and the recovery time after inactivation was prolonged as τ value from (63.23 ± 9.32) to (98.95 ± 10.74), (109.56 ± 13.42) and (127.39 ± 16.13) respectively,P<0.05. Conclusion: Ghrelin may accelerate ICa-L inactivation and prolong the recovery time after inactivation. Ghrelin inhibits ICa-L in a dose-dependent manner.