CAJ | 학술논문

目的：评价无患子皂苷的急性毒性和遗传毒性，为无患子皂苷的日常应用提供毒理学依据。方法通过急性经口毒性试验、哺乳动物红细胞微核试验、细菌回复突变试验和体外哺乳动物细胞染色体畸变试验，检测无患子皂苷对原核生物和真核生物在基因水平和染色体水平的诱变性。结果无患子皂苷的雌雄小鼠急性经口毒性LD50均为4640 mg/kg，可见流涎和黏液便等中毒体征。小鼠骨髓细胞微核试验各剂量组与阴性对照组比较，差异均无统计学意义（ P>0.05）；Ames试验在加或不加S9各剂量组及菌株条件下的回变菌落数均未超过自发回变菌落数（阴性对照）的2倍以上，未观察到剂量-效应关系；染色体畸变试验无患子皂苷对CHL的IC50为75μg/mL，各剂量组与阴性对照组比较，差异均无统计学意义（ P>0.05）。在上述各试验中，阳性对照组与阴性对照组比较，差异均有统计学意义（ P<0.01）。结论在本实验条件下，无患子皂苷不具有遗传毒性。
목적：평개무환자조감적급성독성화유전독성，위무환자조감적일상응용제공독이학의거。방법통과급성경구독성시험、포유동물홍세포미핵시험、세균회복돌변시험화체외포유동물세포염색체기변시험，검측무환자조감대원핵생물화진핵생물재기인수평화염색체수평적유변성。결과무환자조감적자웅소서급성경구독성LD50균위4640 mg/kg，가견류연화점액편등중독체정。소서골수세포미핵시험각제량조여음성대조조비교，차이균무통계학의의（ P>0.05）；Ames시험재가혹불가S9각제량조급균주조건하적회변균락수균미초과자발회변균락수（음성대조）적2배이상，미관찰도제량-효응관계；염색체기변시험무환자조감대CHL적IC50위75μg/mL，각제량조여음성대조조비교，차이균무통계학의의（ P>0.05）。재상술각시험중，양성대조조여음성대조조비교，차이균유통계학의의（ P<0.01）。결론재본실험조건하，무환자조감불구유유전독성。
Objective To evaluate acute toxicity and genotoxicity of sapindus saponin and to provide toxicological basis for sapindus saponin ’s daily applications. Methods Acute oral toxicity test,mammalian erythocyte micronucleus test, bacterial reverse mutation test and in vitro mammalian chromosome aberration test were used to investigate the effect of the sapindus saponin on gene mutation and chromosome aberration in both prokaryotic and eukaryotic cells. Results The acute toxicity test showed that the LD50 of sapindus saponin was 4640 mg/kg for both male and female mice. Toxic symptoms were observed including salivation,mucus and other toxic manifestations. There was no significant difference between the each dose group and the negative control group in the results of mammalian erythocyte micronucleus test( P>0. 05). The results of bacterial reverse mutation test were also negative. In each dose group and strain with or without S9,the number of revertant colonies did not exceed 2 times than that of spontaneous revertant colonies( negative control). No dose-response relationship was observed. The vitro mammalian chromosome aberration test showed that the IC50 of sapindus saponin on CHL was 75 μg/ml,and the differences between each dose group and the negative control group were not statistically significant( P >0. 05 ). However,the positive control group differed from the negative control group in all tests( P <0. 01). Conclusion Under this experimental condition,sapindus saponin has no genotoxicity.