CAJ | 학술논문

目的：研究胺碘酮的剂量和性别及其交互效应对大鼠甲状腺组织功能的影响。方法雌雄各半的 SD 大鼠随机分为3组：对照组和低、高剂量（50，200 mg? kg-1? d-1）胺碘酮实验组（均 n ＝20），给药15周。分别于给药第4，8，12，15周末取血，检测游离 T3（FT3）、游离 T4（FT4）和促甲状腺激素（TSH）水平；称取甲状腺重量并计算脏器系数，同时测定甲状腺 Bcl -2蛋白的表达和Caspase -3的活性，进行甲状腺病理组织检查。结果胺碘酮导致 FT3水平下降，与剂量呈正相关，且在12周水平降至最低点。低剂量胺碘酮实验组的 FT4水平较对照组轻度上升，而高剂量胺碘酮实验组的 FT4水平均小于对照组（第4周除外）。 FT3水平与性别呈正相关，与剂量呈负相关；而 FT4水平与性别、剂量均无线性相关。高剂量胺碘酮实验组的 Bcl -2蛋白表达均有不同程度的增加。结论胺碘酮剂量水平和性别存在交互作用，雄性更易出现甲状腺功能减退。
목적：연구알전동적제량화성별급기교호효응대대서갑상선조직공능적영향。방법자웅각반적 SD 대서수궤분위3조：대조조화저、고제량（50，200 mg? kg-1? d-1）알전동실험조（균 n ＝20），급약15주。분별우급약제4，8，12，15주말취혈，검측유리 T3（FT3）、유리 T4（FT4）화촉갑상선격소（TSH）수평；칭취갑상선중량병계산장기계수，동시측정갑상선 Bcl -2단백적표체화Caspase -3적활성，진행갑상선병리조직검사。결과알전동도치 FT3수평하강，여제량정정상관，차재12주수평강지최저점。저제량알전동실험조적 FT4수평교대조조경도상승，이고제량알전동실험조적 FT4수평균소우대조조（제4주제외）。 FT3수평여성별정정상관，여제량정부상관；이 FT4수평여성별、제량균무선성상관。고제량알전동실험조적 Bcl -2단백표체균유불동정도적증가。결론알전동제량수평화성별존재교호작용，웅성경역출현갑상선공능감퇴。
Objective To investigate the effect of amiodarone dosage level and gender and its interaction on thyroid function in rats .Methods Sixty SD rats, half male and half female, were randomly divided into three groups, with 20 in each group: control group, low and high dosages (50,200 mg? kg-1? d-1 ) amiodarone groups, administered 15 weeks. Free triiodothyronine (FT3), free thyroxine (FT4) and thyroid stimula-ting hormone(TSH) were measured at the 4th,8th,12nd and 15th week. All the rats were sacrificed, thyroid weight, thyroid weight /body weight coefficient.The protein expression of Bcl -2 and the activity of caspase -3, optical microscope pathologic and electron microscope examination were performed.Results With regard to the control group, FT3 level decreased with the dosage of amiodarone increasing , reaching the lowest in the 12nd week.FT4 level sligltly increased in low dosage group, but was lower than control group in high dosage group other than the 4th week.Gender and dosage factors had ineractional effect on FT 3 level, gender was positively correlation with FT3 level, but had no influ-ence on FT4 level.The dosage was negatively correlation with FT3 level. The positively expression of Bcl -2 in high dosage group.The his-topathologic examination found the rupture of thyroid fossil and the enlargementof endoplasmic reticulum, cavitation and so on in low dosage and high dosage group.Conclusion The hypothyroidism was caused by amiodarone in a dosage -dependent manner. Gender and the dosage have interactional effect .Males are more likely to become hypothyroidism after amiodarone adminstration.